SET7 UHRF1 DNA Use for regulating DNA damage repair by SET7-mediated UHRF1 methylation

摘要

The present invention relates to the use of DNA damage repair control through SET7 mediated UHRF1 methylation. UHRF1 is a major epigenetic regulator of DNA methylation maintenance and heterochromatin formation, and the role of UHRF1 in DNA damage repair has been recently emphasized, but the mechanism of regulation of UHRF1 is still unclear. In the present invention, it was found that UHRF1 is methylated by SET7 and demethylated by LSD1. In addition, it was confirmed that methylation of UHRF1 was induced in response to DNA damage, and phosphorylation in S group was necessary for the interaction with SET7. In addition, UHRF1 methylation promoted the conjugation of polyubiquitin chains to PCNA and promoted homologous recombination for DNA repair. In addition, SET7-mediated UHRF1 methylation has been shown to be essential for cell viability against DNA damage. Accordingly, in the present invention, the mechanism of regulation according to the methylation status of UHRF1 by SET7 and LSD1 in the double-helix damage repair pathway was revealed.