Based on single photosynthetic bacterial cell in a batch reactor for bio-production of H2, a model was established for transport of glucose across membrane and production of hydrogen. The concentration distribution of glucose and hydrogen in the bioreactor was estimated, and the effect of light wavelength and intensity on glucose concentration in outside of cells as well as concentration profiles of hydrogen was investigated. The results showed that substantial agreement was achieved between the experimental results and model prediction values; and at wavelength and intensity of light 590 nm and 8000 lx respectively, the concentration of glucose was the lowest, and concentration of hydrogen was the highest outside cells.
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