高纯胸腺肽α1的制备

摘要

为了获得高纯产品,在制备胸腺肽α1 (Tα1)工艺中分别对合成、纯化两步骤进行控制.在固相多肽合成过程中,以TBTU/HOBt/DIEA体系作为缩合试剂,在易形成α螺旋和β转角的肽序位置,通过采用HOBt/DIC补投的方法促使偶联反应完全.在分离纯化阶段,结合反相色谱和离子色谱的优势,以反相离子对液相色谱法为主要分离手段,通过多步分离实现纯化.获得的粗肽纯度为67.2％,胸腺肽α1产品纯度达99.5％以上,总收率为17.2％.此法可作为制备高纯多肽药物的有效手段.%The synthesis and purification steps were controlled in preparation of thymosin α1 to obtain the product with high-purity.In the solid-phase peptide synthesis (SPPS) process,TBTU/HOBt/DIEA was employed as the coupling reagent.In the sites where α-helix or β-turn structure formed easily,re-coupling reagent of HOBt/DIC was used to ensure a complete reaction.In the separation and purification process,a multi-step method was employed,in which reversed-phase ion-pair liquid chromatography was used as the main means,combining the advantages of reversed-phase chromatography and ion chromatography.The results showed that through the re-coupling the crude peptide had a purity of 67.2％ with the use of TBTU/HOBt/DIEA reagent in SPPS.The purity of the refined thymosin α1 was above 99.5％ with a total yield of 17.2％ with the reversed-phase ion-pair liquid chromatography.This separation process avoids complex chromatography separation process.The method may be an efficient way for preparation of peptides with high purity.