无定形介孔磷酸锆固定葡萄糖氧化酶的直接电化学

摘要

Glucose oxidase (GOD)/zirconium phosphate(ZrP)/glassy carbon(GC) electrode was prepared by adsorbing GOD on the amorphous mesoporous zirconium phosphate. The electrocatalytic properties of GOD/ZrP/GC electrode were characterized by cyclic voltammetric method at the medium of phosphate buffer (0.1 mol·L?1). The results showed that there were quicker electron transfer rate and larger surface coverage when GOD was supported on zirconium phosphate. At the same time, the electrochemical device with this electrode showed faster current response and higher sensitivity in detection of glucose, indicating that zirconium phosphate could be more suitable support for immobilization of GOD and achieve better performance of direct electrochemistry.%利用无定形介孔磷酸锆(ZrP)为载体,通过吸附法固定葡萄糖氧化酶(GOD),修饰玻碳(GC)电极得到GOD/ZrP/GC电极.在0.1 mol·L?1磷酸盐缓冲溶液中,利用循环伏安法研究了GOD/ZrP/GC电极的直接电化学行为和对葡萄糖的催化性能.结果表明,无定形磷酸锆ZrP为载体修饰的电极GOD/ZrP/GC其电化学反应电子转移速率快、表观覆盖量大;对葡萄糖的检测表现出较快的电流响应和较高的灵敏度,说明无定形磷酸锆更有利于GOD的固定和酶电极的直接电化学.