充血性心力衰竭患者外周血中内皮素受体基因的表达及意义

摘要

目的 研究内皮素受体基因(ETA、ETB)在充血性心力衰竭(简称心衰)患者外周血中的表达,探讨其临床意义,并建立外周血ETA、ETB基因的逆转录聚合酶链反应(RT-PCR)检测方法.方法 在48例心衰患者和25例健康对照组中分别采用放免法测定血浆内皮素(ET-1)水平,应用RT-PCR技术检测外周血中ET受体基因表达水平.结果 48例心衰患者中,心功能Ⅱ级组(13例)的血浆ET-1水平及ETA 、ETB基因表达与健康对照组比较,两组差异无统计学意义(P>0.05).心功能Ⅲ级+Ⅳ级组(35例)的血浆ET-1水平与健康对照组比较,两组差异具有统计学意义(F=8.014,P<0.01).心功能Ⅲ级+Ⅳ级组的ETA、ETB基因表达与健康对照组比较,两组差异具有统计学意义(F=8.317,P<0.01;F=6.235,P<0.05).结论 心衰(心功能Ⅲ级+Ⅳ级组)患者ETA、ETB的基因表达水平明显高于健康对照组,提示内皮素受体基因可能与心衰的发生、发展密切相关.%Objective To study the expression and significance of endothelin receptor gene in patients with congestive heart fail ure (CHF) ,and to develop the method to measure gene endothelin receptor A (ETA) and endothelin receptor B(ETB) by retrovirus polymerase chain reaction(RT-PCR) in peripheral blood.Methods 48 CHF patients were enrolled,and 25 healthy volunteers were selected as the control group.Plasma endothelin (ET-1) was assessed by radioimmunoassay,and the expression of gene endothelin receptor was measured by RT-PCR.Results There was no statistically significant difference in ET-1 , ETA and ETB expression be tween NYHA ciass Ⅱ (13 case) and the control group.Alternatively,compared with the control group, the indices of ET-1 , ETA and ETB in NYHA class Ⅲ and NYHA class Ⅳ patients(35 case) were significantiy increased.Conclusion There is closely rela tionship between endothelin receptor gene and the occurrence and development of congestive heart failure.