中期因子与血管内皮生长因子C在胰腺肿瘤组织中的表达及其意义

摘要

Objective To explore expression and significance of Midkine(MK) and vascular endothelial growth factor-C(VEGFC) in pancreatic carcinoma.Methods Streptavidin-perosidase(SP) immunohistochemistry was employed to detect expression of MK and VEGF-C in tissues of pancreatic carcinoma, carcinoma adjacent tissues and chronic pancreatitis.A contingency table was created to conduct x2 test and the Spearman Rank Correlation was used to investigate the relationship between the expression of MK and VEGF-C and clinicopathologic factors such as age and gender of patients.size and location of tumor,lymph nodes metastasis and clinical TNM stages.Results The positive expressions of MK and VEGF-C in terms of the number and percentage of their specimens in pancreatic carcinoma tissues were notably higher than their expressions in carcinoma adjacent tissues and chronic pancreatitis tissues(P＜0.05).The differences in the expression of MK and VEGF-C in pancreatic tissues of patients with different clinical TNM stages, regional or non-regional lymph node metastasis were found to be statistically significant(P＜0.05).The expression of VEGF-C in panceatic carcinoma tissues was observed to have positive correlation with that of MK(r=0.68,P＜0.05).Conclusion MK and VEGF-C may be used as indicators for early diagnosis of pancreatic carcinoma, estimating tumor progressionand metastasis.%目的 探讨中期因子(MK)与血管内皮生长因子C(VEGF-C) 在胰腺癌中的表达及其意义.方法 采用免疫组织化学SP法,检测MK及VEGF-C在胰腺癌组织、癌旁组织及慢性胰腺炎组织中的表达情况.采用行×列表进行χ2检验,Spearman等级相关分析MK及VEGF-C的表达与患者年龄、性别、肿瘤部位、淋巴结转移、TNM临床分期等临床病理因素之间的关系.结果 MK与VEGF-C在胰腺癌组织中的阳性表达例数及所占百分比明显高于其在癌旁组织及慢性胰腺炎组织的表达(P＜0.05).在不同TNM临床分期以及有无区域淋巴结转移患者的胰腺组织中MK及VEGF-C表达差异有统计学意义(P＜0.05).胰腺癌组织中VEGF-C与MK表达正相关(r=0.68,P＜0.05).结论 MK及VEGF-C可能成为胰腺癌早期诊断、判断肿瘤进展和转移的指标.