EphA2、CD34、D2-40在卵巢上皮性肿瘤中的表达及其与临床病理的关系

摘要

目的 检测EphA2、CD34、D2-40在卵巢上皮性肿瘤中的表达,探讨其与临床病理因素的关系.方法 采用免疫组化方法 检测10例正常卵巢上皮、48例良性卵巢上皮性肿瘤、47例交界性卵巢上皮性肿瘤以及205例恶性卵巢上皮性肿瘤组织中EphA2、CD34及D2-40的表达,并计数微血管密度(MVD)及微淋巴管密度(LMVD).结果 (1)正常卵巢组织中EphA2、CD34-MVD、D2-40-LMVD的表达较卵巢上皮性肿瘤组织显著降低,差异有统计学意义(χ2=14.662 4,P=0.002).(2)恶性卵巢上皮性卵巢肿瘤组织中EphA2的表达、CD34-MVD、D2-40-LMVD与肿瘤的临床分期、分化程度、有无淋巴结转移有关,差异有统计学意义(P＜0.05),但与患者年龄无关(P＞0.05).(3)恶性卵巢上皮性肿瘤组织中EphA2不同表达程度组间CD34-MVD、D2-40-LMVD差异有统计学意义(F=4.33,P=0.005 5;F=7.91,P=0.000 1).EphA2的表达与CD34-MVD、D2-40-LMVD呈正相关(r=0.220 4,P=0.001 5;r=0.297 8,P=0.000 0).且在恶性卵巢上皮性肿瘤组织中CD34-MVD与D2-40-LMVD呈正相关(r=0.534 0,P=0.000).结论 EphA2的过表达一方面可以促进肿瘤细胞的增殖能力,另一方面可以促进肿瘤组织中新血管及新淋巴管的产生,新生的血管及淋巴管在肿瘤细胞的侵袭及转移过程中起着相辅相成的作用.%Objective To examine the expressions of EphA2 , CD34 and D2-40 in ovarian epithelial neoplasms, and investigatetheir relationships with clinicalpathologh.Methods SP immunohistochemical staining was used to examine the expressions of E-phA2 , CD34 and D2-40 in 10 normal ovarian specimens , 48 benigh ovarian specimens,47 boundary ovarian specimens and 205 malig-nant ovarian specimens.Results (1) The expressions of EphA2 ,CD34 and D2-40 in normal ovarian tissues were higher than in o-varian epithelial neoplasms(P＜0.01).(2) The expressions of CD34MVD,D2-40-LMVD in the maglignant ovarian epithelial neo-plasms were positively related to clinical stage, differential and lymph node metastasis (P＜0.05).But no statistically difference wasfound between them with the patients＇ age( P＞ 0.05 ) .( 3) A statistically difference of MVD and LMVD were found between the differentgrade expressions of EphA2 in malignant Ovarian(P＜0.01).And the expressions of EphA2 were positively correlated with CD34-MVDand D2-40-LMVD(P＜0.01).The expressions of CD34-MVD and D2-40-LMVD were positively correlated with each other in malignantneoplasms(P＜0.01).Conclusion EphA2 overexpression on the one hand could promote the proliferation of tumor cells.