新生大鼠持续缺氧条件下水通道蛋白4与脑水肿的关系

摘要

Objective To determine the relationship of AQP4 and brain edema under the condition of continue hypoxia in newborn rat. Methods Healthy and 10-day-old SD rats were randomly divided into control and experimental groups.Experimental group, the right common carotid artery ligation and hypoxia at different times into 2 h,4 h,8 h,16 h four sub-groups, the control group were sham-operated. Observed each animal nervous behavior change,and killed them at corresponding time points,hematoxy-lin-eosin staining to observe rat hippocampal CA1 region morphology, and calculated the relative expression of AQP4 by immuno-histochemistry and fluorescence quantitative PCR. Results The experimental group showed different degrees of hypoxia symptoms; the experimental groups,compared with control group,had different degree of edema,and the degree of edema was serious with the extension of time,neurons exhibited irreversible damage;the expression of the AQP4 protein and AQP4 RNA were related downward trend. Conclusion The reduced expression of AQP4 participates the formation of cerebral edema in the neonatal rat hypoxic-ischemic conditions.%目的 探讨水通道蛋白4(AQP4)在新生大鼠持续缺氧过程中与脑水肿的关系.方法 健康10 d龄SD大鼠,随机分为对照组和实验组.实验组结扎右侧颈总动脉,然后缺氧不同时间分为缺氧缺血(HI)2 h组、HI 4 h组、HI 8 h组、HI 16 h组4个亚组,对照组行假手术.观察每组动物神经行为学改变,各组实验取脑组织,行苏木精伊红(HE)染色、免疫组织化学染色、荧光定量PCR,观察新生大鼠海马CA1区形态变化和AQP4表达水平.结果 实验组均出现不同程度的缺氧症状;实验组与对照组比较,有不同程度的水肿,且随着时间的延长,水肿加重,神经元呈现不可逆损伤;AQP4蛋白和RNA的表达水平呈下降趋势.结论 AQP4表达水平下降参与新生大鼠持续缺氧缺血条件下脑水肿的形成.