哮喘患者外周血Th17细胞水平及功能的研究

摘要

目的：探讨T h17细胞在哮喘发病机制中的作用。方法以10例哮喘患者和10例健康志愿者为研究对象，分别留取外周血标本。利用RT-PCR技术检测外周血中淋巴细胞所表达的维甲酸相关较孤儿受体γt（RORγt） mRNA的相对量，流式细胞仪检测外周血中Th17细胞的比例，ELISA法检测血浆中白细胞介素（IL）-17的水平。结果 RORγt mRNA在哮喘组的表达水平（0．46±0．07）高于对照组（0．15±0．02），差异有统计学意义（ P＜0．01）。T h17细胞在哮喘组的比例（28．53±7．20）％高于对照组（14．72±2．33）％，差异有统计学意义（P＜0．01）。IL-17在哮喘组的水平（102．31±11．45）pg/mL 明显高于对照组（59．68±8．85）pg/m L。结论 T h17细胞与哮喘具有相关性，可能通过分泌以IL-17为主的细胞因子来上调炎症反应，加重哮喘病情。%Objective To explore the role of Th17 cells in the pathogenesis of asthma .Methods Peripheral blood was obtained from 10 patients with asthma(asthma group) and 10 healthy volunteers(control group) .RORγt mRNA expression of lymphocytes was measured by RT-PCR ,percentage of Th17 cell was detected by FCM ,IL-17 in plasma was examined by ELISA .Results RORγt mRNA level in asthma group(0 .46 ± 0 .07) was significantly higher than that in control group (0 .15 ± 0 .02) ,proportion of Th17 cell in asthma group(28 .53 ± 7 .20)% was significantly higher than that in control group(14 .72 ± 2 .33)% ,P<0 .01 .Com-pared with IL-17 level in control group(59 .68 ± 8 .85)pg/mL ,there was significant increase in asthma group(102 .31 ± 11 .45)pg/mL(P<0 .01) .Conclusion Th17 cell is associated with asthma ,and probably aggravating asthma condition through increasing in-flammation secreting IL-17 .