目的：探讨右美托咪啶（Dex ）与咪达唑仑多次镇静对幼年大鼠神经细胞损伤及成年后学习记忆功能的影响。方法：采用随机数字表法，将36只雄性SD大鼠随机分为3组（n＝12）：对照组（C组）、咪达唑仑组（M组）和Dex组（D组）。C组大鼠每日皮下注射生理盐水0．1 mL/kg 。M组大鼠每日皮下注射咪达唑仑2．4 mg/kg（0．1 mL/kg）。D组大鼠每日皮下注射Dex 75μg/kg（0．1 mL/kg）。各组大鼠每日注药1次，共进行3 d。于用药结束后6 h取血浆测定S100β蛋白，神经元特异性烯醇化酶（NSE）含量。于用药结束后2月，采用Morris水迷宫实验检测大鼠成年后学习记忆能力。结果与C组比较，M 组成年后水迷宫实验逃逸潜伏期延长，穿越原平台次数减少。用药结束后6 h ，血清S100β蛋白，NSE表达上升，差异有统计学意义（P＜0．01）。D组各项指标变化无显著性。结论：相比于咪达唑仑，新型麻醉镇静药Dex对幼年动物神经细胞损伤及成年后学习记忆功能的影响较小。%Objective To investigate the influence of multiple sadation of dexmedetomidine (Dex) and midazolam on young rats′nervous injury and the learning and memory ability after grow up .Methods 36 male SD rats were randomly allocated to 3 groups (n=12):control group(C) ,midazolam group (M ) and dexmedetomidine group(D) .The group C was given normal saline 0 .1 mL/kg by subcutaneous injection .The group M was subcutaneously injected by midazolam 2 .4 mg/kg ,0 .1 mL/kg .The group D was subcutaneously injected by dexmedetomidine 75 μg/kg ,0 .1 mL/kg .Each group received once injection for 3 d .Plasma S100β,NSE were measured at 6 h after medication .The rats′memory ability after grow up was assessed by the Morris water maze test .Results Compared with the group C ,the escape latency in the group M was extended and the times across the platform was decreased . The expressions of S100βand NSE in the group M were enhanced at 6 h after medication .There was no statistical difference be-tween the group C and D .The changes of various indexes in the group D had no significance .Conclusion Compared with midazo-lam ,using new type narcotic and sedative agent dexmedetomidine had little influence on the young rat nervous injury and the learn-ing and memory ability after grow up in rat .
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