孕晚期用HBIG对HBV携带孕妇胎盘Toll样受体的影响

摘要

目的 探讨孕晚期应用乙型肝炎免疫球蛋白(HBIG)干预对乙型肝炎病毒(HBV)携带孕妇胎盘组织TLR3、TLR4、TLR7表达的影响.方法 收集2010年6～12月在该院产检并分娩的HBsAg(+)孕妇53例,正常孕妇10例.血清HBV-Ms和胎盘TLR3、TLR4、TLR7平均光密度分别采用ELISA和Envision免疫组织化学法检测.结果 孕妇血清HBsAg(+)组,胎盘TLR3、TLR7的表达水平均较对照组高;胎盘HBsAg(-)组与胎盘HBsAg(+)组间,TLR3、TLR7表达水平比较差异无统计学意义;孕晚期使用HBIG者,胎盘中TLR3、TLR7的表达与未应用HBIG者比较差异无统计学意义.结论 尚不能认为孕期应用HBIG可改变胎盘的TLR3、TLR7的表达.%Objective To explore the influence on placental Toll-like receptor 3 (TLR3 ), TLR4, TLR7 in the hepatitis B surface antigen( HBsAg )positive women with the usage of Hepatitis B Immune Globulin (HBIG) during the late pregnancy. Methods 53 HBsAg positive and 10 normal pregnant women recruited in this study were regularly performed perinatal care in our hospital from June to Dec. 2010. Serum hepatitis B virus markers (HBV-Ms),average optical density of placental TLR3, TLR4 and TLR7 were respectively detected by ELISA and Envision immunohistochemistry. Results The expressions of placental TLR3 and TLR7 in HBsAg positive gravidas were stronger than those of normal ones. The expressions of placental TLR3 and TLR7 were not statistically different between placental HBsAg positive and negtive gravidas. Neither were the HBIG users or not during the late pregnancy. Conclusion Placental TLR3 and TLR7, perhaps, join in the immunoregulation of anti-HBV transmission. The maternal HBsAg positive can stimulate the expression of placental TLR3, 7. The influence on placental TLR3 and TLR7 of HBsAg carriers with the usage of HBIG during late pregnancy is yet to be researched into.