Netrin-1及VEGF在食管鳞癌组织中的表达水平及其相关性研究

摘要

Objective To detect the expression of Netrin-1 protein and VEGF protein in squamous cell carcinoma(ESCC) tissue and its correlation research.Methods The expressions of Netrin-1 protein and mRNA,VEGF and mRNA in 50 cases of ESCC,19 cases of adjacent atypical hyperplasia tissue and 20 cases of normal tissue were detected using immunohistocheistry and in situ hybridization.Results The expressions of Netrin-1 protein and mRNA,VEGF and mRNA were positive in ESCC tissue.The posive rate of Netrin-1 protein and mRNA,VEGF and mRNA in ESCC tissue was significantly higher than those of atypical hyperplasia tissue(P＜0.05) ;in metastatic group,the positive rates of Netrin-1 protein and mRNA,VEGF in ESCC tissue and atypical hyperplasia tissue was higher than those in non-metastatic group(P＜0.05) ;VEGF and Netrin-1both between positively correlated(r=0.941 3,P＜0.01),VEGF mRNA and Netrin-1mRNA both between positively correlated(r=0.584 7,P＜0.01),and Netrin-1both between positively correlated(r=0.941 3,P＜0.01).Conclusion The expression of Netrin-1 protein and mRNA,VEGF in human ESCC tissue is increased.VEGF and Netrin-1 expression in esophageal cancer lymph node metastasis plays a role.Netrin-1 and VEGF is related to development,invasion and metastasis of ESCC.%目的 检测食管鳞癌组织中神经轴突导向因子-1(Netrin-1)及血管内皮细胞生长因子(VEGF)的表达水平,探讨Netrin-1和VEGF的相关性.方法 应用免疫组织化学SP法和原位杂交方法检测50例食管鳞癌组织及其相应的19例癌旁不典型增生组织和20例正常食管黏膜组织中VEGF、Netrin-1蛋白及mRNA的表达水平.结果 (1)食管鳞癌组织中Netrin-1、VEGF蛋白及mRNA阳性表达率均高于癌旁不典型增生组织和正常食管黏膜组织(P＜0.05)；(2)有淋巴结转移组食管鳞癌组织中Netrin-1、VEGF蛋白及mRNA阳性表达率均高于无淋巴结转移者(P＜0.05)；(3)食管鳞癌组织中VEGF和Netrin-1阳性表达率间呈正相关(r=0.941 3,P＜0.01),VEGF mRNA和Netrin-1 mRNA阳性表达率间呈正相关(r=0.584 7,P＜0.01).结论 人食管鳞癌组织中VEGF、Netrin-1蛋白及mRNA均呈高表达；Netrin-1、VEGF的表达在食管癌的淋巴结转移过程中起一定的作用；且二者存在正相关,可能共同参与食管癌的发生、发展.