白花蛇舌草对人骨肉瘤MG-63细胞Bax基因表达的影响

摘要

Objective To investigate the effect of hedyotic diffusa willd injection on osteosarcoma MG‐63 cells Bax gene expres‐sion .Methods MTT was be used to detect the inhibition rate of MG‐63 cells by hedyotic diffusa willd injection after 6 ,12 ,24 ,48 h certain concentration ,RT‐PCR was be used to detect the intracellular expression of Bax gene .Results Hedyotic diffusa willd injec‐tion can significantly inhibit the proliferation of MG‐63 cells ,as the concentration was 100 μL/mL ,Bax gene expression was signifi‐cantly increased over time(P<0 .05) .Conclusion Upregulating the expression of Bax gene by hedyotic diffusa willd injection can induced human osteosarcoma M G‐63 cells apoptosis to death .%目的：探讨白花蛇舌草对人骨肉瘤M G‐63细胞Bax基因表达的影响。方法采用M T T法检测一定浓度白花蛇舌草注射液对MG‐63细胞作用6、12、24、48 h后的细胞增殖抑制率，实时荧光PCR（RT‐PCR）检测细胞内Bax基因的表达情况。结果白花蛇舌草注射液能明显抑制MG‐63细胞增殖，浓度为100μL／mL时，随着作用时间的延长，Bax基因表达明显升高（P＜0．05）。结论白花蛇舌草注射液可能通过上调Bax基因表达诱导人骨肉瘤M G‐63细胞凋亡。