人细胞分裂控制蛋白4在口腔鳞状细胞癌中的表达及临床意义

摘要

目的：检测人细胞分裂控制蛋白4（hCDC4）在口腔鳞状细胞癌（OSCC）中的表达情况，分析其与临床病理参数之间的相关性。方法收集52例手术切除的 OSCC 组织及12例正常组织，运用免疫组织化学技术检测 hCDC4表达情况，分析 hC‐DC4蛋白表达与临床病理参数之间的相关性。应用 hCDC4‐siRNA 瞬时转染 OSCC 细胞系 Tca8113，M TT 及蛋白免疫印迹（Western blot）检测细胞增殖能力及 c‐Myc 和 Cyclin E 蛋白表达变化。结果 hCDC4蛋白在正常组织中的表达显著高于对应的OSCC 组织（83．3％ vs ．25．0％，P＜0．05）；hCDC4蛋白低表达与较大肿瘤直径（≥4 cm）和高临床分期（Ⅲ＋Ⅳ）显著相关（P ＜0．05）；敲低 Tca8113细胞中 hCDC4表达，导致细胞增殖能力显著增强（P＜0．05）以及 c‐Myc 和 Cyclin E 蛋白蓄积。结论 hC‐DC4表达缺失导致 c‐Myc 和 Cyclin E 蛋白蓄积可能是促进肿瘤进展的重要因素。%Objective To investigate human cell division control protein 4(hCDC4) expression and its correlation with the clini‐copathological features in oral squamous cell carcinoma(OSCC) .Methods We freshly collected 52 samples of surgically resected OSCC tissues and 12 samples of normal tissues .hCDC4 expression in the samples was detected by immunohistochemical staining . The correlation between hCDC4 protein expression and clinicopathological feature was analysed .OSCC cells and Tca8113 were transfected with hCDC4‐siRNA ,cell proliferation and c‐Myc and Cyclin E protein expression were determined by using M TT and Western blot .Results The hCDC4 protein expression in normal tissues was significantly up‐regulated compared to those in OSCC tissues (83 .3% vs .25 .0% ,P < 0 .05) .Clinicopathological analysis revealed that reduced hCDC4 expression was associated with large tumor size ( ≥ 4 cm) and high clinic stage ( Ⅲ + Ⅳ ) (P< 0 .05) .hCDC4 knockdown by siRNA led to increased cell prolifera‐tion and c‐Myc and Cyclin E protein accumulation in Tca8113 cells .Conclusion Loss of hCDC4 may promote tumor progression by resulting in c‐Myc and Cyclin E protein accumulation in OSCC .