目的：探讨建立家兔纤维化模型的CCl4最佳剂量。方法将50只健康雄性家兔随机分为5组，每组10只，分别用0．2、0．5、1．0、2．0、5．0 mL/kg剂量的纯CCl4对1～5组家兔进行皮下注射（每周2次）建立肝纤维化模型，并于注药后4、8、12周在B超引导下行肝穿刺病理活检，对比研究不同剂量C C l4建立家兔肝纤维化模型的效果。结果家兔死亡多发生在3周之内，5周之后趋于稳定。至12周，1～5组家兔存活数及肝纤维化成模率分别为：8只（60％）、7只（70％）、3只（40％）、1只（20％）、0只（10％）。结论当CCl4的给予剂量大于1．0 mL/kg时易导致家兔急性中毒而死亡；皮下注射0．5 mL/kg剂量的纯CCl4，家兔死亡率低，肝纤维化成模率高。%Objective To explore the optimal dose of carbon tetrachloride(CCl4 ) in establishment of rabbit model of hepatic fi-brosis .Methods A total of 50 healthy male rabbits were randomly and averagely divided into 5 groups .Then established rabbit modelofhepaticfibrosisbysubcutaneousinjectingdifferentdose(0.2,0.5,1.0,2.0,5.0mL/kg)ofpureCCl4 ,twiceaweek.Ultra-sound-guided liver puncture pathological examination were performed at 4 ,8 and 12 weeks after injection ,and the effect of different doses of CCl4 were comparative studied .Results Death of the models usually happened within 3 weeks after first injection and be-came stable after 5 weeks .To 12 weeks ,the number of survived rabbits of 1 to 5 groups were 8 ,7 ,3 ,1 ,0 respectively ,and the suc-cess rate of the liver fibrosis were 60% ,70% ,40% ,20% ,10% respectively .Conclusion The rabbits prone to acute poisoning and death when CCl4 given doses are greater than 1 .0 mL/kg ;Subcutaneous injection of pure CCl4 ,at a dose of 0 .5 mL/kg ,twice a week ,can establish rabbit model of hepatic fibrosis with low mortality and high success rate .
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