目的：观察调节性T细胞（T reg ）在严重烧伤大鼠肠道淋巴结中的表达变化，探讨其变化对肠道淋巴结其他 T淋巴细胞的影响及其与肠道内毒素移位的关系。方法取雄性SD大鼠50只，分成正常对照组和烧伤后0．5、1、2、4 h组（各10只），对各烫伤组大鼠背部造成30％全身体表面积Ⅲ度烧伤；分离肠道淋巴结，采用流式细胞仪技术检测肠道淋巴结 T reg、CD3＋CD4＋/CD3＋CD8＋淋巴细胞的表达变化；采用鲎试剂（动态浊度法）检测门静脉血浆内毒素水平。结果烧伤后大鼠肠道 T reg表达与CD3＋CD4＋/CD3＋CD8＋淋巴细胞比值呈负相关（r＝-0．827，P＜0．01）；而 Treg水平与门静脉血浆 ET 水平呈正相关（ r＝0．782，P＜0．01）。结论严重烧伤大鼠肠道淋巴结T reg表达的增加，对肠道其他T淋巴细胞有免疫抑制作用，与肠道内毒素移位有着密切的关系。T reg在严重烧伤后肠道免疫屏障中可能起重要的作用。%Objective To observe the change of regulatory T cells expression in severely burned rats gut ,To investigate the effects of regulatory T cells on CD3+CD4+ /CD3+CD8+lymphocytes and its relation with gut-origin endotoxin translocation .Meth-ods Fifty SD male rats were randomly divided into normal control group(n=10)and burn model groups(n=40) .Rats were burned to achieve Ⅲ degree scalding ,and a 30% total body surface area(TBSA) burn model was made .Rats were sacrificed before(normal control group) and after 0 .5 ,1 ,2 ,4 burn hour(PBH groups) .Flow cytometry techniques were used for the detection of the expres-sions of regulatory T cells and CD3+CD4+ /CD3+CD8+ lymphocyte in intestinal lymph nodes which were separated .The dynamic turbidity method was used for detection of endotoxin levels in portal vein blood .Results The expression of regulatory T cells was negativelycorrelatedwithCD3+CD4+ /CD3+CD8+ lymphocyteratio(r= -0.827,P<0.01)inintestinallymphnodeofrats,while the regulatory T cells was positively correlated with ET levels in portal vein blood plasma .(r=0 .782 ,P<0 .01) .Conclusion The expression of the regulatory T cells in intestinal lymph node in severely burned rats was increased compared to that in normal con-trol group .Regulatory T cells suppressed the expression of intestinal T lymphocytes ,leading to gut immune inhibition .The translo-cation of intestinal endotoxin has a close relationship with regulatory T cells in severely burned rats .Regulatory T cells could have portal effects on intestinal immunity barrier .
展开▼
