依赖基质细胞衍生因子-1对心肌梗死血管生成及功能恢复的作用分析

摘要

目的：研究依赖基质细胞衍生因子‐1（SDF‐1）对心肌梗死血管生成及功能恢复的作用。方法将大鼠随机分成检测组（n＝65）和处理组（n＝64），其中检测组在心肌梗死前后的1、2、4、7、14、28 d分别随机处死5只大鼠（梗死组），进行SDF‐1蛋白水平测定，同时设置5只大鼠为假手术对照。处理组的64只大鼠另随机分成4个小组，每组16只：分别为梗死＋ SDF‐1组、梗死＋抗SDF‐1组、梗死＋生理盐水组、正常＋生理盐水组。对各组进行相应处理后，对比心肌梗死后的SDF‐1蛋白水平情况，对比各组骨髓间质干细胞（MSCs）归巢量及新生血管的密度水平，对比各组大鼠的心肌缩短分数（EF）和射血分数（FS）变化水平。结果梗死组的SDF‐1水平在1～7 d后显著高于假手术组，并达到峰值水平，随后逐渐下降，在14 d时恢复至正常，差异有统计学意义（P＜0．05）。经过SDF‐1处理后的组别MSCs归巢量和新生血管的密度水平显著高于其他各组，梗死＋抗SDF‐1组的水平则明显低于梗死＋生理盐水组的水平，差异有统计学意义（均 P＜0．05）。梗死＋SDF‐1组大鼠的心功能改善情况显著优于其他各组，差异有统计学意义（P＜0．05）。结论 SDF‐1可促使心肌梗死血管生成，改善心功能。%Objective To study the role of dependence of stromal cell derived factor‐1(SDF‐1) on myocardial infarction an‐giogenesis and function recovery .Methods The rats were randomly divided into the test group (65 cases) and the treatment group (64 cases) ,in which 5 rats in the test group were randomly killed on 1 ,2 ,4 ,7 ,14 ,28 d before and after myocardial infarction(infarc‐tion group) and the SDF‐1 protein level was detected .At the same time the sham operation group(5 cases) was set up;64 rats in the treatment group were randomly divided into four groups ,16 cases in each group ,which were infarction+SDF‐1 group ,infarct+anti‐SDF‐1 group ,infarct+normal saline group and normal+normal saline group .After the corresponding processing in each group ,the SDF‐1 levels after myocardial infarction ,MSCs homing amount ,neovascularization density ,EF and FS levels were compared among various groups .Results The SDF‐1 level after 1-7 d in the infarction group was significantly higher than that in the sham opera‐tion group ,and reached a peak level ,then decreased gradually and restored to normal on 14 d ,the difference was statistically signifi‐cant (P<0 .05) .The MSCs homing amount and neovascularization density level in the SDF‐1 treatment groups were significantly higher than those in other groups ,which in the infarction+anti‐SDF‐1 group were significantly lower than those in the infarction+normal saline group ,the differences were statistically significant (all P<0 .05) .The cardiac function improvement situation in the infarction +SDF‐1 group was significantly better than that in other groups ,the difference was statistically significant (P<0 .05) . Conclusion SDF‐1 can promote angiogenesis in myocardial infarction ,also improve the heart function .