ANTIBODY-DEPENDENT CELL-MEDIATED PHAGOCYTOSIS ASSAY FOR RELIABLY MEASURING UPTAKE OF AGGREGATED PROTEINS

摘要

The present disclosure provides methods of assaying for antibody-dependent cell-mediated phagocytosis (ADCP). In some embodiments, the methods include monomerizing and labeling a protein, contacting the protein with a protein-specific antibody to form an antibody-protein complex, contacting the antibody-protein complex with a phagocytic cell to permit phagocytosis, and assessing the amount of internalized fluorescence.