结合黏附分子家族A表达水平与鼻咽癌细胞株放疗敏感性的关系研究

摘要

Objective The radiotherapy resistance is one of important causes for nasopharyngeal carcinoma(NPC) treatment failure.Junctional adhesion molecule A(JAMA)is closely correlated with the tumor poor prognosis.Thus this experiment is to in vestigate the relationship between JAMA expression and the radiosensitivity of NPC.Methods To overexpress or interfere the JAMA expression in CNE2 and HONE1 cell lines.Then different doses of X-ray were adopted to conduct irradiation.The cell clone formation capacity and cellular apoptosis change were detected after 24 h.The role of JAMA in the NPC radiotherapy was understand.The related signal pathway protein in cell lines with different JAMA expression was detected by Western blot.Results The cell lines with low JAMA expression were more sensitive to radiotherapy:After low JAMA expression,the D0 value in the CNE2 cell line was decreased from 3.26 ±0.78 to 1.92 ± 0.23;the Dq value was decreased from 46.51 ± 4.27 to 32.12 ± 3.19.The radio therapy induced apoptosis was significantly increased in the cell lines with low JAMA expression,after low JAMA expressing,thcellular apoptosis was elevated from 6.9 ％ ± 0.9 ％ to 13.7 ％ ± 1.3 ％;the HONE1 cellular apoptosis was elevated from 6.5 ％ + 1.1 ％ to 12.3 ％ ± 1.7％;JAMA overexpression cell lines were significantly decreased.The preliminary mechanism research results showed that JAMA played the effect via Akt signal pathway.Conclusion This research results verifiy that JAMA expression level is closely correlated with the radiosensitivity of NPC cell line:JAMA can increase the radiotherapy resistance of NPC cell lines,which provides a new feasible research direction for NPC enhancing radiosensitivity.%目的 研究结合黏附分子家族A(JAMA)表达水平与鼻咽癌细胞株放疗敏感性的关系.方法 过表达或干扰CNE2和HONE1细胞株中JAMA的表达,然后采用不同剂量X射线进行照射,24 h后检测细胞克隆形成能力及细胞凋亡变化,了解JAMA在鼻咽癌放疗中的作用.通过Western blot检测JAMA不同表达水平细胞株放疗后的相关信号通路蛋白.结果 JAMA低表达细胞株对放疗更敏感:JAMA低表达后,D0值在CNE2细胞株中从3.26±0.78下降为1.92±0.23;Dq值从46.51±4.27下降至32.12±3.19.放疗诱导的凋亡在JAMA低表达细胞株中显著增加,JAMA低表达后,细胞凋亡率从6.9％±0.9％上升至13.7％±1.3％;HONE1细胞凋亡率从6.5％±1.1％上升至12.3％±1.7％;JAMA过表达细胞株中显著减少.结论 JAMA表达水平与鼻咽癌细胞株放疗敏感性密切相关,JAMA能增加鼻咽癌细胞株放疗抵抗.