Effect of Green Valley Lingzhi on Monocyte Adhesion to Endothelium and Adhesion Molecule Expression

摘要

This research studied the effect of sera derived from rats administrated Green Valley Lingzhi (GVL) on low- density lipoprotein (LDL) oxidation, monocyte adhesion to the human endothelial cells line ECV304 (EC) and the expression of adhesion molecules induced by oxidized-LDL (ox-LDL) and glycated albumin. LDL oxidation was determined by measuring the TBA- reactive substances in the supernatants expressed as malondialdehyde equivalents (nmol/100μL medium). Monocyte adhesion to the EC was determined by measuring myeloperoxidase activity of adherent monocyte. The expression of ICAM-1 and VCAM-1 was determined by cell ELISA and flow cytometric technique. The sera derived from the rats administrated 0.12g/kg of GVL once (0.5h, 1h, 2h, 3h after administration) or two times (0.5h, 1h after administration) were not exhibited significant effects on LDL oxidation, but the sera from the rats administrated 0.12g/kg (2h and 3h after administration) or 0.12g/kg, 0.24g/kg and 0.72g/kg GVL sera (p. o for 10 days) showed the significant effects on reducing LDL oxidation (P<0.05); GVL sera (0.12g/kg, 0.24g/kg and 0.72g/kg GL for 10 days) inhibited monocyte adhesion to EC induced by AGE(P<0.05), and 0.24g/kg and 0.72g/kg inhibited monocyte adhesion to EC induced by ox-LDL(P<0.05). The results by cell ELISA method showed that the GL sera (0.24g/kg, 0.72g/kg and 0.72g/kg for 10 days) produced significant effects of inhibiting the expression of ICAM-1 induced by ox-LDL and expression of ICAM-1 and VCAM-1 induced by glycated albumin. GVL decreased LDL oxidation, inhibited monocyte adhesion to the EC by decreasing the expression of adhesion molecules.