Fascin-1蛋白在结直肠癌中的表达及其临床意义

摘要

目的：探讨Fascin‐1蛋白在结直肠癌中的表达及其临床意义。方法采用免疫组化MaxVision两步法检测87例结直肠癌和28例癌旁正常组织中Fascin‐1蛋白的表达情况，分析Fascin‐1蛋白表达与结直肠癌临床病理特征的关系。Kaplan‐Meier和Cox回归模型分析Fascin‐1蛋白表达与结直肠患者生存的相关性。结果 Fascin‐1在结直肠癌组织和癌旁正常组织中的阳性表达率分别为43．7％（38／87）和7．1％（2／28），二者差异有统计学意义（P＜0．05）。Fascin‐1蛋白表达在不同年龄、TNM分期、淋巴结转移及远处转移间差异均有统计学意义（P＜0．05），但是在不同性别、病理类型、肿瘤位置、分化程度间差异无统计学意义（P＞0．05）；Kaplan‐Meier分析显示Fascin‐1蛋白高表达组患者的总生存率明显低于低表达组（P＝0．009）。多因素Cox回归分析表明Fascin‐1蛋白高表达是影响结直肠癌患者预后的独立危险因素（相对危险度[HR]＝2．087，95％ CI：1．196～3．642， P＝0．010）。结论 Fascin‐1蛋白在结直肠癌组织中呈高表达，其与患者的远期生存率相关。Fascin‐1蛋白可能是影响结直肠癌患者预后的独立危险因素。%Objective To investigate the expression of Fascin‐1 in colorectal cancer (CRC) and its clinical significance .Meth‐ods Immunohistochemistry MaxVision method was adopted to detect the protein expression of Fascin‐1 in 87 CRC tissues and 28 para‐cancer tissues ,and analyzed the expressions of them and the relations to clinicopathologic characteristics .Kaplan‐Meier plots and Cox proportional hazards regression model were used to analyze the prognostic value of Fascin‐1 expression .Results The posi‐tive rates of Fascin‐1 expression in CRC tissues and para‐cancer tissues were 43 .7% (38/87) and 7 .1% (2/28) respectively ,with significant difference (P<0 .05) .Fascin‐1 expression was correlated with age ,TNM staging ,regional lymph node metastasis and distant metastasis ,with significant difference (P<0 .05) ,whereas it was not correlated with gender ,tumor location ,pathologic dif‐ferentiation and pathology type ,with no statistical difference(P>0 .05) .In the Kaplan‐Meier analysis ,patients with Fascin‐1 over‐expression had significantly shorter overall survival than those patients with negative Fascin‐1 expression (P=0 .009) .Multivari‐able analysis by Cox regression model further showed that Fascin‐1 over‐expression was a significantly independent predictor of poorer overall survival (HR=2 .087;95% CI:1 .196-3 .642 ,P=0 .010) .Conclusion The expression of Fascin‐1 is high and its re‐lated to the long‐term survival time of patients with CRC .It is an independent prognostic factor for CRC .