基质金属蛋白酶-1基因多态性对汉族人群牙种植早期失败的影响

摘要

目的 从遗传学的角度探讨基质金属蛋白酶-1(MMP-1)的基因多态性是否与种植体早期失败有关.方法 采用病例对照的临床试验设计方法,将选取的53例不吸烟、无牙周炎的汉族种植患者根据有无种植体早期失败(1个月内)分为失败种植体组和成功种植体组;同时收集所有患者的静脉血提取DNA;采用聚合酶链式反应和限制性片段长度多态性方法对MMP-1-1607位点进行基因型的测定.结果 携带MMP-1-1607基因型1G/2G杂合子的患者其种植体早期失败的发生率明显高于携带MMP-1-1607基因型1G/1G纯合子患者.多元Logistic回归分析结果显示:MMP-1-1607 1G/2G基因型相对于MMP-1-16071G/1G基因型的比值比是13.894(P＜0.05).结论 MMP-1-1607 1G/2G基因型和种植体早期失败有相关性.%Objective To make clear the relationship between the genetic polymorphism of MMP-1 and implant early failure.Methods We conducted a case control study,53 cases patients (no smoking history and without periodontitis) who have underwent dental implant were divided into experimental group(with dental implant failure) and control group(with dental implant success) according to early failure of dental implant (within one month).DNA of 53 patients in Han nationality were extracted by using Chelex-100 method and polymorphisms of the mmp-1-1607 gene were detected by restriction fragment length polymorphism after polymerase chain reactions.Results Patients with MMP-1-1607(1G/2G) genotype exhibited a significantly higher occurrence of implant early failure than those with MMP-1-1607(1G/1G) gene type(P＜0.05).The OR of the 1G/2G versus the 1G/1G of the MMP-1-1607 genotype was 13.894(P＜0.05).Conclusion MMP-1-1607(1G/2G) genotype is associated with implant early failure.