STUDY ON THE MECHANISM OF INTERFERON ACTION (Ⅺ)——EFFECT OF pppA2’p5’A2’p5’A ON THE LEVEL OF cAMP AND cGMP IN MACROPHAGES

摘要

In this paper, the increase of cellular cAMP and cGMP levels in macrophages induced bypppA2’p5’A2’p5’A （briefly 2’-5’P₃A₃） is first reported. The optimal concentration of 2’-5’P₃A₃ for the elevation of cellular cGMP to the highest level is 10-7-10-6mol/L, while thatfor cAMP is 10-7mol/L. The time for cGMP to reach its peak value is 15 min and that forcAMP is 2 h, when the cells are treated with 2’-5’ P₃A₃ at 10-7mol/L, which is the optimalconcentration for developing biological effect of macrophages （phagocytosis）. These resultssuggest that cGMP and cAMP may be related to, or may be the mediators for, 2’-5’P₃A₃action.