重组质粒白细胞介素-24对人宫颈癌Hela细胞裸鼠移植瘤的生长及血管生成的抑制作用研究

摘要

Objective To determine the inhibitory effects of recombinant interleukin IL-24 plasmid on growth of human cervical carcinoma Hela cell xenograft tumor and angiogenesis in nude mice. Methods Human cervical carcinoma cell lines, the Hela cells, were injected into four-week-old nude mice to establish human cervical carcinoma xenograft tumor model. The nude mice were then assigned into phosphate buffer solution (PBS) group, blank-plasmid group and recombinant IL-24 plasmid group and were subject to PBS, pDC316 plus Lipofectamine 2000, and pDC316 plus hIL-24 Lipofectamine 2000 intratumor injection, respectively. The volume of xenograft tumor and mi-crovascular growth among each group were compared. Results IL-24 gene was successfully transfected and expressed in xenograft tumor cells in IL-24 group. There was statistical difference in the mass of xenograft tumor among groups (P0.05). Furthermore, higher micro vascular density was revealed in PBS and blank-plasmid groups than that in recombinant IL-24 group (P0.05). Conclusion IL-24 suppresses the growth of human cervical carcinoma Hela cells xenograft tumor in nude mice. The inhibitory effect on angiogenesis might be one of the anti-tumorigenesis mechanisms.%目的 探讨基因重组质粒白细胞介素(IL)-24对人宫颈癌Hela细胞裸鼠移植瘤的生长及血管生成的抑制作用.方法 对4周龄裸鼠注射人宫颈癌细胞株Hela细胞建立人宫颈癌裸鼠移植瘤模型,把造模成功的裸鼠分为磷酸盐缓冲液(PBS)、空载质粒、基因重组质粒IL-24 3组,分别向瘤内注射PBS、pDC316+ Lipofec-tamine 2000、pDC316-hIL-24+Lipofectamine 2000,比较干预后各组移植瘤的体积及微血管生成的情况.结果 IL-24基因成功转染入实验组肿瘤细胞并在其中成功表达,移植瘤的质量组间比较差异有统计学意义(P＜0.01),PBS组、空载质粒组的移植瘤质量高于基因重组IL-24组(P＜0.01),PBS组、空载质粒组移植瘤质量差异无统计学意义(P＞0.05);PBS组、空载质粒组微血管密度显著高于重组IL-24组(P＜0.01),但PBS、空载质粒组间比较差异无统计学意义(P＞0.05).结论 IL-24能够抑制宫颈癌Hela细胞裸鼠移植瘤的生长,抑制肿瘤新生血管生长是抑制肿瘤生长的机制之一.