RECOMBINANT PLASMID DNA PJ DB (MSIL), ENCODING OF HUMAN INTERLEUKIN-2 SYNTHESIS IN YEAST CELLS SACCHAROMYCES CEREVISIAE, METHOD OF ITS PREPARATION, AND YEAST STRAIN SACCHAROMYCES CEREVISIAE - A PRODUCER OF HUMAN INTERLEUKIN-2

摘要

The invention relates to biotechnology. The aim of the invention is to increase the yield of interleukin-2 in yeast cells at the expense of constructing a recombinant plasmid DNA pjDB (MSIL), the development process of its designing and producing strain transformed with this plasmid. The plasmid contains the yeast LEU 2 fragment and the yeast DNA dvumikronnoy gene. ensure the maintenance of its stable in yeast high copy number; gene coding region of a human interleukin-2, flanked by the promoter and transcription terminator gene RN05 yeast, as well as fragments of bacterial DNA, permitting replication of the plasmid in E.coli cells and carrying the ampicillin resistance gene. Yeast cells of strain GC-1- GRF18, transformed with the plasmid pjDB (MSIL), produce inYepleykin-2 in an amount on the order of 30-50 mg per liter of yeast culture, which corresponds to 10 units. / Ml. 3 SP f-ly. (LS