目的：探讨核因子（NF）-κB p65亚型和心肌素在子宫肌瘤发生中的作用机制。方法蛋白印迹法检测29例子宫肌瘤和相应肌层组织以及15例原代子宫肌瘤细胞和相应肌层细胞中NF-κB p65、心肌素的表达；RNA干扰技术抑制子宫肌瘤细胞中NF-κB p65基因表达，光镜观察细胞生长状况，蛋白印迹法检测干扰后子宫肌瘤细胞中NF-κB p65和心肌素的表达。结果与正常肌层相比，子宫肌瘤组织和细胞中的NF-κB p65相对表达量增高（P＜0．05），而心肌素的相对表达量降低（P＜0．05）。 RNA干扰后，与未转染的子宫肌瘤细胞相比， NF-κB p65的相对表达量降低（P＜0．05），而心肌素的表达量相对增高（P＜0．05）。结论 NF-κB p65与心肌素可能相互作用共同参与了子宫肌瘤的形成。%Objective To explore the mechanisms of NF-κB p65 and myocardin in the pathogenesis of uterine leiomyoma. Methods Western blotting was employed to examine the expression of NF-κB p65 and myocardin in u-terine leiomyoma tissues (n=29) and the primary uterine leiomyoma smooth muscle cells and myometrium cells (n=15). The RNAi assay was applied to suppress the expression of NF-κB p65 in uterine leiomyoma smooth muscle cells, which allowed for observation under optical microscopy. The expression of NF-κB p65 and myocardin was examined by using Western blotting assay following RNAi. Results Compared with normal myometrium, the uterine leiomyoma tissues and cells were associated with heightened NF-κB p65 (P<0.05) and attenuated myocardin expression (both P<0.05). RNA interference resulted in attenuated of NF-κB p65 and augmented myocardin expression (both P<0.05) compared with uterine leiomyoma smooth muscle cells without transfection. Conclusion The interaction between NF-κB p65 and myocardin may have played a role in the pathogenesis of uterine leiomayoma.
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