目的建立IMCD细胞单层H+/K+交换的测定方法。方法采用BCECF/AM荧光探针法,测定不同浓度CO2培养的IMCD细胞H+/K+交换。结果呼酸组的酸负荷后[pH]i恢复率为0.0620±0.012,高于对照组(0.0504±0.009,P0.05)。结论荧光探针法测定原代培养家兔肾脏IMCD细胞H+/K+交换,具有操作简便、稳定可靠、经济安全等优点。%AIM To establish the fluorescent measurement technique for H+/K+ exchange in primary cultured IMCD cell monolayers of rabbit kidney. METHODS To measure H+/K+ exchange in the primary cultured IMCD cell monolayers under different CO2 concentrations by the BCECF/AM fluorescent probe.RESULTS [pH]i recovery rate after acid loaded in the respiratory acidosis group was 0.0620±0.012,and was significantly higher than that of the control group(0.0504±0.009,P0.05). CONCLUSION The fluorescent measurements of H+/K+ exchange in primary cultured IMCD cell monolayers of rabbit kidney has many benefits which are convenient manipulation, stable and credit result, economy and safety , this method should further be popularized.
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