目的 怒是影响人类健康的重要情志因素,分为愤怒和郁怒两种表达方式,两者诱发病证的机制尚未完全明确.该文主要探索愤怒、郁怒情志反应的微观机制.方法 采用居住入侵和社会隔离的方法制备愤怒、郁怒反应大鼠模型,并分别予以调肝方药经前平颗粒、经前舒颗粒干预,以正常大鼠作为对照,分别取愤怒、郁怒模型组以及经前平、经前舒给药组大鼠的海马用以基因芯片检测,初步筛选出与愤怒、郁怒密切相关的基因5-HTR2C、GABABR2和5-HTR3B,运用RT-PCR和Western blot技术仅对海马中基因5-HTR2C进一步进行检测.结果 与正常组相比,5-HTR2C mRNA和蛋白表达在愤怒、郁怒反应大鼠海马中均增高(P<0.01),而且愤怒组的改变程度明显大于郁怒组,上述改变在模型用药组均得到不同程度的改善,基本可恢复到正常水平.结论 愤怒、郁怒情志刺激可导致海马基因5-HTR2C的表达水平改变,调肝方药在一定程度上可缓解上述情况,说明5-HTR2C与怒密切相关,且与愤怒关系更加密切,以上结果为下一步的研究提供方向和线索.%Aim Anger is an important emotion response affecting human health, which is divided into anger-in and anger-out.But the mechanism of disease and syndrome induced both anger-in and anger-out is not yet fully clear.So the aim of the paper is to explore micro-mechanism of disease and syndrome induced both anger-in and anger-out.Methods The anger-in and anger-out animal models were prepared using a social isolation plus resident-intruder stress.The rats were treated by" tiao gan fang yao"Jingqianping granule and Jingqianshu granule.Normal rats as control.Genes closely related with anger-in and anger-out were initially screened utilizing Gene chip to detect hippocampus of other groups.The genes were 5-HTR2C, GABABR2 and 5-HTR3B.Futher 5-HTR2C in hippocampus moving was detected by RT-PCR and Western blot in the paper.Results Compared with normal group, the expressions of 5-HTR2C mRNA and protein of both anger-in and anger-out animal model were significantly higher in hippocampal( P ＜ 0.01 ).Meanwhile, the change of anger-out animal model group was more significant than that of anger-in animal model group.These changes were improved to normal levels in different degrees after administrating drugs.Conclusions The emotion responses of anger-in/anger-out can lead to changes of gene expression of 5-HTR2C in the hippocampal.In addition, to some extent." tiao gan fang yao can improve the above.The results illustrate the close relationship between 5-HTR2C and anger, closer with anger-out.These results provide direction and clues for further research.
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