豹皮樟总黄酮调控瘦素、TGF-β1对肝纤维化大鼠的预防作用研究

摘要

Aim To evaluate whether Litsea Coreana level ( TFLC ) would have regulatory effects on the expressions of Leptin and TGF-β1 of hepatic fibrosis in rats. Methods The liver fibrosis model rats were induced by injecting 50% CCl4( 1 ml · kg-1) twice a week for 12 weeks. From the first week, all the therapeutic groups were treated with TFLC (100, 200, 400 mg · kg-1 ) and the colchicines ( 0. 1 mg · kg-1) respectively. At the end of the twelfth week, the levels of ALT, AST, HA, LN, PINP, CIV, Collagen I, Leptin, TGF-β in serum and Hyp in liver were measured. Masson staining was used to evaluate the degree of hepatic fibrosis. The mRNA and protein expressions of Ob-Rb, TGF-βR1 and Smad3 in liver were detected by RT-PCR and Western blot. Results TFLC ( 200 and 400 mg · kg-1) treatment significantly reduced the　elevations of ALT, AST, HA, LN, PINP, CIV, Collagen I , Leptin, TGF-β1 in serum and Hyp in liver. In addition, TFLC treatment improved the morphologic changes of hepatic fibrosis, suppressed expressions of Ob-Rb, TGF-βR1, Smad3 in the liver fibrosis in rats. Conclusions TFLC is able to ameliorate liver injury and protect rats from liver fibrosis. This process may be related to inhibiting the expression of Ob-Rb, TGF-βR1 and Smad3.%目的 研究豹皮樟总黄酮(TFLC)调控瘦素(leptin)和转化生长因子-β1 (TGF-β1)对肝纤维化大鼠的预防作用.方法 采用CCl4诱导大鼠肝纤维化模型,TFLC灌胃给药第12周末,检测ALT、AST及HA、LN、PⅢNP、CⅣ含量;检测Ⅰ型胶原(Collagen Ⅰ)、瘦素、TGF-β1的含量;检测Hyp及组织病理变化;检测瘦素受体(OB-Rb)、TGFβ1Ⅰ型受体(TGFβR1)、Smad3 mRNA及蛋白表达.结果 TFLC(200、400 g·L-1)能明显降低肝纤维化大鼠肝纤维化指标及血清Ⅰ型胶原、瘦素、TGF-β1水平;降低Ob-Rb、TGFβR1、Smad3的mRNA及蛋白表达.结论 TFLC能有效预防CCl4诱导的大鼠肝纤维化,可能与下调肝内瘦素及其受体,抑制TGF-β1/Smad通路有关.