基于UPLC-TOF-MS研究冰片对丹参酮ⅡA在大鼠体内药代动力学的影响

摘要

目的：运用超高效液相色谱/四级杆飞行时间质谱( ultra performance liquid chromatography time-of-flight mass spectrometer)技术,研究口服不同剂量的冰片对丹参酮ⅡA大鼠体内药代动力学的影响。方法大鼠分别口服丹参酮IIA及丹参酮IIA加冰片混悬液,用UPLC-TOF-MS方法检测丹参酮IIA的血药浓度,用DAS软件拟合,计算相关药动学参数。结果检测丹参酮IIA在血浆中0.6~0.01 mg·L-1线性良好(r=0.999,n=7)。不同剂量的冰片与丹参酮ⅡA联合使用可明显增大丹参酮ⅡA的血药浓度( Cmax ),同时药时曲线下面积(AUC)明显增加,特别是15 mg·kg-1冰片对丹参酮ⅡA药代动力学产生明显影响。结论冰片与丹参酮ⅡA的配伍合用可以明显提高丹参酮ⅡA的吸收,增加丹参酮ⅡA的口服生物利用度,表明复方丹参中丹参和冰片配伍的科学性,显示二者相须相使的配伍关系。%Aim An ultra performance liquid chroma-tography coupled with time-of-fight mass spectrometry ( UPLC-TOF-MS) technique was used to evaluate the effect of borneol on the pharmacokinetics of tanshinone IIA in rats after oral administration of tanshinone IIA with different doses of borneol. Methods The rats were orally administered with tanshinone IIA and the mixed suspension of tanshinone IIA coupled with bor-neol. Respectively, the concentrations of tanshinoneIIA were determined by an UPLC-TOF-MS method, and the pharmacokinetic parameters were calculated by the software of DAS. Results The good linearity range of tanshinone IIA in plasma was 0 . 6~0 . 01 mg ·L-1(r=0.999,n=7). The compatibility of differ-ent doses of borneol and tanshinone IIA could increase the blood concentrations of tanshinone IIA ( Cmax ) , in the area under the plasma concentration-time curve ( AUC) increased significantly, especially when 15 mg ·kg-1 of borneol was orally administered with tanshi-none IIA. Conclusions Borneol is able to obviously enhance the absorption and the relative bioavailability of tanshinone IIA. The described herb-drug interac-tions might prove the scientific rationality of the com-patible ratio of traditional Chinese medicine.