基于蛋白芯片技术探讨丁氏溃结灌肠液对结肠炎大鼠肠道组织差异蛋白表达的影响

摘要

Aim To investigate the effect of Ding''s herb enema prescription on intestinal tissue related target in rat colitis induced by dextran sulfate sodium(DSS), and to elucidate the mechanism of Ding''s herb enema prescription in improving the intestinal inflammation and intestinal fibrosis.Methods Rats were fed with 3.5％ DSS.The rats were randomly divided into positive drug group, model group, Control group, and Ding''s herb enema prescription group.The positive drug group was treated with mesalazine enema, and Ding''s herb enema prescription group was treated with Ding''s herb enema prescription.The colon mucosa was taken once a day for 6 weeks.The changes of intestinal inflammatory response and intestinal fibrosis related proteins were detected by GSR-CAA-67 antibody protein array, and the differentially expressed proteins were screened out.Results Eight proteins showed statistical differences, including IFN-γ, erythropoietin(EPO), TIMP-2, TIM-1, IL-6, TIMP-1, TNF-α, IL-22 (P0.05).Conclusions Ding''s herb enema prescription has the effect of multiple targets, which may improve the intestinal inflammatory response and intestinal fibrosis to achieve the purpose of treatment of ulcerative colitis(UC).%目的 基于蛋白芯片技术探讨丁氏溃结灌肠液对结肠炎大鼠肠道组织相关靶点的影响,阐释丁氏溃结灌肠液改善肠道炎症反应及肠纤维化的可能机制.方法 大鼠随机分为阳性药组、模型组、正常组、丁氏溃结灌肠液组,5只/组.除正常组之外,其他3组均给予3.5％硫酸葡聚糖钠(dextran sulfate sodium, DSS)喂养,结肠炎造模成功后,阳性药组使用美沙拉秦灌肠,丁氏溃结灌肠液组使用丁氏溃结灌肠液灌肠,每天1次,连续4周,停药1 d后,取大鼠结肠黏膜组织,采用GSR-CAA-67抗体蛋白芯片技术检测各组大鼠肠道组织炎症反应及肠纤维化相关蛋白的变化,筛选出各组差异性表达的蛋白.结果 通过蛋白芯片检测,发现共有8种有意义的差异蛋白,其中模型组和空白组对照组对比筛选出的8种蛋白包括IFN-γ、促红细胞生成素(erythropoietin, EPO)、TIMP-2、TIM-1、 IL-6、TIMP-1、TNF-α、IL-22,差异均有统计学意义(P0.05).结论 丁氏溃结灌肠液具有多靶点的作用,可能通过改善肠道炎症反应及肠纤维化达到治疗溃疡性结肠炎的目的.