目的：建立同时测定保安万灵丹中升麻素苷、5-O-甲基维斯阿米醇苷、茅术醇、β-桉叶醇和苍术素含量的方法。方法：采用高效液相梯度洗脱法，色谱柱为C18色谱柱（200 mm×4.6 mm，5µm），流动相A为甲醇-乙腈（2∶1），流动相B为0.1%磷酸水溶液，体积流量1.0 mL·min-1，检测波长分别为254 nm（升麻素苷和5-O-甲基维斯阿米醇苷）、203 nm（茅术醇和β-桉叶醇）和340 nm（苍术素）。结果：5种成分线性范围分别为升麻素苷4.30~86.00μg·mL-1（r=0.9994）、5-O-甲基维斯阿米醇苷5.85~117.00μg·mL-1（r=0.9997）、茅术醇16.42~328.40μg·mL-1（r=0.9993）、β-桉叶醇15.16~303.20μg·mL-1（r=0.9999）、苍术素12.78~255.60μg·mL-1（r=0.9995）；精密度、重复性及稳定性试验RSD＜2.0%；平均加样回收率（n=6）分别为升麻素苷97.49%（RSD为1.45%）、5-O-甲基维斯阿米醇苷96.81%（RSD为0.94%）、茅术醇98.60%（RSD为1.65%）、β-桉叶醇97.96%（RSD为1.38%）、苍术素99.22%（RSD为1.24%）。结论：该方法操作简便，结果准确，重复性好，可作为保安万灵丹的含量控制方法。%Objective: To develop a method for the simultaneous content determination of prim-o-glucosylcimifugin, 5-o-methylvisammioside, hinesol,β-eudesmol and atractylodin in Bao'an Wanling pills. Methods:The gradient elution HPLC method was adopted. The quantitative analysis was carried out on the C18 column (200 mm×4.6 mm, 5 µm). A linear elution of acetonitrile-methanol (2∶1) and 0.1% phosphoric acid solution were adopted as the mobile phase at the lfow rate of 1.0 mL·min-1. The detection wavelength was 254 nm for prim-o-glucosylcimifugin and 5-o-methylvisammioside, 203 nm for hinesol andβ-eudesmol, 340 nm for atractylodin.Results:The linear ranges of prim-o-glucosylcimifugin, 5-o-methylvisammioside, hinesol,β-eudesmol and atractylodin fell within the ranges of 4.30-86.00 μg·mL-1(r=0.9994), 5.85-117.00 μg·mL-1 (r=0.9997), 16.42-328.40 μg·mL-1(r=0.9993), 15.16-303.20 μg·mL-1 (r=0.9999), and 12.78-255.60μg·mL-1(r=0.9995), respectively. RSDs for precision, stability and repetition tests were all<2.0%. The average recoveries and RSDs were 97.49% (1.45%), 96.81% (0.94%), 98.60% (1.65%), 97.96% (1.38%) and 99.22% (1.24%) for the above components, respectively.Concolusion:The method is simple, accurate, and reproducible and can be used in the simultaneous content determination of prim-o-glucosylcimifugin, 5-o-methylvisammioside, hinesol,β-eudesmol and atractylodin in Bao'an Wanling pills.
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