鼠伤寒沙门氏菌porin诱导BALB／crn小鼠细胞免疫的研究

摘要

目的探讨鼠伤寒沙门氏菌porin(STM-porin)诱导BALB／c小鼠细胞免疫的能力。方法迟发型变态反应(DTH)、IL-2分别采用足垫肿胀、MTT法；利用尼龙毛柱纯化免疫的BALB／c小鼠的T淋巴细胞通过尾静脉转移到非免疫小鼠，再用500LD50的STM攻击。结果用STM-porin免疫的BALB／c小鼠可以产生较高水平的DTH(3.6±0.2，P＜0.01)和IL-2(26.2±4.9，P＜0.01)；同时T淋巴细胞被动免疫保护(42.9％)也明显高于LPS组(0％)，P＜0.01。结论这些结果说明STM-porin可能诱导BALB／c小鼠产生较强的细胞免疫。%Aim To investigate cell mediated-immunity induced by porin of Salmonella typhimurium(STM). Methods Level of delayed-type hypersensitivity(DTH), IL-2 of immunized BALB/c mice were determined by footpad swelling,MTT and transfered protective rate of T cells purified by neloy wool were studied with 500LD50 of the bacteria(intraperitoneally). Results A marked level of DTH(3. 6±0. 2,P＜0. 01),IL-2(26.2±4.9,P＜0. 01) and the immuinized T cells transfer protec tive level (42.9％ ,P＜0. 01) was induced by porin from STM. Conclusion These results indicated that porin of STM might in duced stronger CMI in BALB/c mice.