小鼠感染猪附红细胞体后Th17相关因子的实验研究

摘要

To investigate the variation of interleukin-17(IL-17) and transforming growth factor-β(TGF-β)in peripheral blood in mouse, BALB/c mice infected with Mycoplasma suis were used t constituteaa model.The variation of IL-17 and TGFβ concentration in peripheral blood of mouse were detected by using the (method of ELISA after 1, 3,5,7 and 9 days, respectively.The results showed that after one day, three days, five days, seven days and nine days, IL-17 in the peripheral blood of mouse infected with Mycoplasma suis was significantly higher than those in the control group(P＜0.01) respectively.Five days later, IL-17 concentration in the peripheral blood of mouse raised to the highest point(P＜0.01).After one day, three days, five days and seven days, TGF-β in the peripheral blood of mouse infected with Mycoplasma suis was respectively significantly higher than those in the control group (P＜ 0.01 ).Three days later, TGF-β concentration in the peripheral blood of mouse raised to the highest point(P＜0.01).Nine days later, TGF-β concentration in the peripheral blood of mouse infected with Mycoplasma suis was no statistically significant difference compared to the control group(P＞0.05).In conclusion, IL-17 and TGF-β in peripheral blood of BALB/c mice infected with Mycoplasma suis increased, and raised to the highest point in the fifth day and third day.The results prouide scientific data for pro-inflammatory mechanism of Mycoplasma suis and provided a theoretical basis for clinical immunotherapy.%目的 检测BALB/c小鼠感染猪附红细胞体后外周血中白细胞介素-17(IL-17)、β转化生长因子(TGF-β)的变化.方法 用猪附红细胞体阳性血感染BALB/c小鼠1d、3d、5d、7d、9d后,ELISA方法检测小鼠外周血中IL-17、TGF-β水平变化.结果 小鼠感染猪附红细胞体后的第1d、3d、5d、7d、9d外周血中IL-17水平分别高于对照组,差异有统计学意义(P＜0.01),且第5d,外周血IL-17水平达到高峰;感染后的第1d、3d、5d、7d外周血中TGF-β水平分别高于对照组,且在第3d,外周血中TGF-β水平达到高峰,第9d,TGF-β水平与对照组相比无差异(P＞0.05).结论 小鼠感染猪附红细胞体后出现IL-17、TGF-β水平升高的现象,且分别在第5d、第3d出现高峰.该研究结果为附红细胞体病的致炎机制提供科研数据,并为临床免疫治疗提供理论依据.