2007-2011年宁夏地区沙门氏菌分布特征分析

摘要

目的 对宁夏地区2007-2011年沙门氏菌分布特征和流行菌株的分子分型研究.方法 使用法国梅里埃公司的API 20E生化鉴定条对56株沙门氏菌进行鉴定,依据沙门氏菌属Kauffmann-White-Le Minor抗原表用沙门氏菌属诊断血清确定血清型.参照中国细菌性传染病分子分型实验室监测网络(PulseNet China)的沙门氏菌脉冲场凝胶电泳(PFGE)分子分型方法 对宁夏地区33株肠炎沙门氏菌进行基因分型,用BioNumerics(5.1)软件聚类分析.结果 宁夏地区5市检出的56株沙门氏菌有14个血清型,除固原市之外的4市常见血清型都为肠炎沙门氏菌,33株肠炎沙门氏菌分为11个PFGE带型.结论 宁夏不同地区不同来源的沙门氏菌血清型分布有较大差异,肠炎沙门氏菌为流行菌株.%The purpose of this study is to investigate the characteristics of Salmonella serotype distribution and PFGE patterns isolated from different areas of Ningxia, to control Sahnonella infection effectively, and to manage the problem of food poisoning caused by Sahnonella infection. Fifty-six strains were tested by the API 20E bacterial identification system,and then the serotype was detected by the Kauffman-White Salmonella's antigen schema. The above strains were classified by pulsed field gel electrophoresis (PFGE) analysis. Results showed that among the 56 strains of Salmonella 14 serotypes were detected, of which Sahnonella enteritidis was the main strain. The 33 strains of Sahnonella enteritidis were classified into 11 different genotypes by PFGE analysis. It indicates the characteristics of Salmonella serotype distribution is different in various areas of Ningxia, among which the Salmonella enlerilidis is the epidemic strain.