宁波野生鼠类携带的沙粒病毒检测和种系进化分析

摘要

To detect and phylogeneticaly analyze arenavirus carried by wild rodents in Ningbo ,China ,two pairs of degener‐ate‐primers were designed to amplify the S and L gene of arenavirus ,and then RT‐PCR was applied to detect arenavirus carried by rodents which captured from Ningbo port area .All 73 rodents samples were detected ,of which 12 Rattus norvegicus were positive ,an arenavirus virus strain named DX1401 were separated .The S gene amplified products of DX1401 was about 413 bp ,and the L gene was 1 204 bp .The phylogenetic analysis of S segments showed that DX1401 strain was in one branch of phylogenetic tree with Mobala virus strain ACAR3080 .The genetic distance to Mobala virus strain ACAR3080 was the closest , with the value of 0 .467 ;the phylogenetic analysis of L segments showed that DX1401 strain were in one group of phylogenetic tree with Lassa virus strain Josiah ,NL ,Z148 ,Bamba‐R114 ,Soromba‐R ,Nig08‐A37 ,Nig08‐A47 ,Mobala virus strain ACAR3080 ,Morogoro virus strain 13017/2004 ,Mopeia virus strain Mozambique ,and AN 21366‐BNI .The genetic distance to Mobala virus strain ACAR3080 was the closest ,with the value of 6 .953 .In conclusion ,the study confirmed the existence of arenavirus popular in wild rodents in Ningbo ,China .%目的：对宁波地区野生鼠类携带的沙粒病毒进行检测并分析病毒基因序列特征。方法设计沙粒病毒L、S基因的简并引物，采用RT‐PCR方法对宁波口岸捕获的鼠类样品进行沙粒病毒检测，分离病毒，对PCR产物进行测序及系统发生分析。结果共检测73份鼠类样品，其中12份褐家鼠经检测为阳性，分离获得沙粒病毒株DX1401，该病毒株S片段扩增片段大小为413 bp ，L片段扩增大小1204 bp。S片段的系统发生分析显示DX1401与Mobala病毒株ACAR3080位于同一分支，与Mobala病毒株ACAR3080遗传距离最为接近，值为0．467；L片段的系统发生分析显示DX1401与Lassa病毒株Jo‐siah、NL、Z148、Bamba‐R114、Soromba‐R、Nig08‐A37、Nig08‐A47，Mobala病毒株 ACAR3080，Morogoro病毒株13017／2004， Mopeia病毒株Mozambique、AN 21366‐BNI位于同一组，与Mobala病毒株ACAR 3080遗传距离最为接近，值为6．953。结论本研究证实了宁波地区野生鼠类中存在沙粒病毒流行。