应用改良凝集试验(MAT)法检测东北地区野生鸟类弓形虫感染的血清学调查

摘要

目的 了解中国东北地区野生鸟类的弓形虫感染情况.方法 在对东北地区野生鸟类进行调查时,从翼根静脉采集约500 μL全血,凝血后分别分离出血清,采用改良凝集试验(MAT)方法检测血清中弓形虫抗体的阳性率.结果 共收集到179份野生鸟类血清样本,其中弓形虫阳性血清样本20份,总的血清阳性率约为11.17％.179只野生鸟隶属于9目17科31种,其中鸽形目、雀形目、隼形目、鬻形目、佛法僧目、雁形目的血清阳性率分别为5.26％(1/19),9.09％(9/99),14.29％(3/21),15.00％(5/22),16.67％(1/6),和25.00％(1/4).按不同食性分类,肉食性、杂食性或水生动植物为食的鸟类的血清阳性率分别为12.00％(3/25),10.60％(15/141),15.38％(2/13).按照鸟类是否迁徙分类,候鸟和留鸟的血清阳性率分别为11.67％(14/120),10.71％(6/59).结论 东北地区的野生鸟类中弓形虫抗体的阳性率约为11.17％,显示弓形虫感染现象常见于野生鸟类.%We detected the seroprevalence of Toxoplasma gondii (T.gondii) in the wild birds in northeast China.The wild bird's blood was collected from the cutaneous ulnar vein and the serum was isolated and used for detection of anti T.gondii antibody by modified agglutination test (MAT).Results showed that totally 179 birds' serum samples were collected.Twenty serum samples (11.17％) were positive with T.gondii antibody,which belonged to 9 orders,17 families and 31 species.The seroprevalence against T.gondii was about 5.26％ (1/19) in Columbiformes,9.09％ (9/99) in Passeriformes,14.29％ (3/21) in Falconiformes,15.00％ (5/22) in Piciformes,16.67％ (1/6) in Coraciiformes,and 25.00％ (1/4) in Anseriformes.Based on their feeding behavior,the seroprevalence was 12.00％ (3/25) in carnivorous wild birds,10.60％ (15/141) in omnivorous wild birds,and 15.38％ (2/13) in the wild birds feeding on aquatic animals or plants.These wild birds also can be sorted as migratory and sedentary (non-migratory) according to their migration habits,and the serum positivity was 11.67％ (14/120),and 10.71％ (6/59) respectively.The seroprevalence against Toxoplasma gondii in wild birds in northeast China is about 11.17％,which indicates a common infection of Toxoplasma gondii in wild birds.