江苏省禽源空肠弯曲杆菌的分离鉴定及耐药性研究

摘要

The aim of the research is to study the prevalence and antimicrobial resistance of Campylabocter jejuni isolated from poultry in Jiangsu Province.A total of 753 samples from poultry meat and cloacal swabs were investigated,after the pure culture and the polymerase chain reaction of the mapA gene,207 isolates were examined for antimicrobial resistance by using K-B method according to World Health Organization.Results showed that all isolates performed different degree of antimicrobial resistance except meropenem,gentamycin,kanamycin,florfenicol and fosfomycin,the resistance rates of 194 strains to trimethoprim,norfloxacin,ceftriaxone and tetracycline were 100％,84.02％,80.9％ and 79.4％ respectively,1 strain isolated from Xuzhou was resistant to 92.6 ％ antibiotics.The multi-drug resistance appeared and the advantage of drug-resistant spectrum was LIN/CTX/CRO/NOR/CIP/T/TE,the resistant type focused on 40％-60％.The research provided evidence for surveillance of the antimicrobial resistance of C.jejuni and highlighted the need to employ more prudent use of critically important antimicrobial.%目的 了解江苏省禽源空肠弯曲杆菌的流行情况及耐药特征.方法 从江苏徐州、淮安和扬州等7市采集禽肉产品和泄殖腔棉拭子样品共753份,分离细菌,PCR方法鉴定空肠弯曲杆菌后,根据WHO推荐的K-B法测定9大类27种抗生素的敏感性.结果 753份样品共分离出空肠弯曲杆菌207株,分离率为27.5％.药敏结果显示,所有菌株除了对美罗培南、庆大霉素、卡那霉素、氟苯尼考和磷霉素保持高度敏感性,对其它抗生素产生不同程度耐药,对磺胺类、喹诺酮类、β-内酰胺类和四环素类抗生素耐药率最高,其中对甲氧苄氨嘧啶、诺氟沙星、头孢曲松和四环素耐药率分别高达100％、84.02％、80.9％和79.4％,一株分离自徐州的菌株对92.6％的抗生素产生耐受性.测试菌株出现多重耐药现象,优势耐药谱为LIN/CTX/CRO/NOR/CIP/T/TE,菌株耐药类型在40％～60％数量最多,各市菌株耐药类型不一.结论 江苏省禽源空肠弯曲杆菌耐药性比较普遍,多重耐药菌株较突出,应严格监管养殖场抗生素的使用及流通阶段食品源空肠弯曲杆菌的监测.