轮状病毒和大肠杆菌不同免疫方式免疫奶牛乳中抗体消长规律的研究

摘要

sixteen pregnant cows during their later gestation were used to be immunized with human Escherichia coli (E.coli) and/or Rotavirus (RV) to induce hyper immune antibodies against human being diarrhea. The immune ways were as follows: one was that different cows were immunized with E.coli or RV, the other was that the same cow was immunized with E.coli and RV at the same time. The antibody levels in milk of cows immunized were detected by In vitro Agglutination and Reverse Indirect Hemagglutination Inhibition Test and their changes were described. The results show that the dynamical changes of hyper immune antibodies against E.coli and RV in milk of cows immunized by two different ways were similar and their high levels of antibodies maintained for two months.%以引起婴儿腹泻的轮状病毒RV和大肠杆菌E.coli免疫妊娠后期的奶牛，使之产生抗这两种病原的抗体。免疫方式分为两种：一种是分别用E. coli和RV作免疫原免疫奶牛，另一种以两者同时免疫奶牛。然后定期采乳，分别以试管凝集反应和反向间接血凝抑制试验检测大肠杆菌抗体和轮状病毒抗体，并比较这两种不同免疫方式免疫奶牛乳中抗体的消长规律。试验结果表明：单独以大肠杆菌或轮状病毒作免疫原与这两者同时作免疫原，免疫奶牛乳中抗体消长规律相似，抗体效价也相近，且均可维持近两个月。