化学发光免疫试剂盒检测牛肝、牛肉中阿维菌素类药物的残留

摘要

To verify the independence R &D of avermectins chemiluminescence immunoassay kit,the method of chemiluminescent microparticle immunoassay and liquid chromatography -mass spectrometry method were used to detect the residues of avermectinsin beef livers and beef muscles,and compared the difference between the results of two methods.The results showed that IC50 value of the kits standard curve was 14.805 ~26.235 ng/L,the recovery of the method was in the range of 95.25% ~104.55%,and the coefficient of variation CV was less than 15%,the limits of detection of the kit for the beef liver and beef muscle were 1.463 ng/kg and 1.392 ng/kg;The results of kit method was well consistent with as the results of LC -MS method.This kit method was efficient, reliable and could meet the on -site rapid detection of animal tissue avermectins residues needs.%为验证自主研发的阿维菌素类药物化学发光免疫试剂盒的检测效果,采用化学发光微粒子免疫法和液相色谱-质谱法对牛肝、牛肉样品中阿维菌素类药物残留量进行检测,并比对2种方法试验结果的差异.结果表明:这种试剂盒标准曲线IC50值的波动范围为14.805~26.235 ng/L,对牛肝、牛肉样品在2、4、8μg/kg 3个水平做加标回收试验,其回收率均在95.25%~104.55%之间,变异系数CV均小于15%,对牛肝、牛肉样品的最低检测限分别为1.463、1.392 ng/kg.这种方法与液相色谱-质谱法检测实际样品的阴、阳性判断结果一致.该方法高效、可靠,可满足现场快速检测动物组织中阿维菌素类药物残留的需要.