AN IMPROVED CHEMILUMINESCENCE IMMUNOASSAY KIT FOR THE DETECTION OF HIV 1 AND HIV 2 ANTIBODIES IN HUMAN SERUM AND PLASMA

摘要

The present invention relates to a chemiluminesccence immuno assay kit for analyzing the presence of HIV 1 and HIV 2 antibodies present in human serum or plasma. More specifically it is a diagnostic kit for the detection of HIV 1 and HIV 2 antibodies present in human serum or plasma. The chemiluminescent immunoassay kit includes a microtiter plate which is immobilized with recombinant HIV1 gp41 antigen or synthetic peptide HIV 1 gp41 or a combination thereof; recombinant HIV 1 C terminus of gp 120 or synthetic peptide of HIV1 C terminus of gp 120 or a combination thereof; recombinant antigen HIV 1 gp 160 or synthetic peptide of HIV 1 gp 160 or a combination thereof; recombinant antigen HIV 1 O sub group or synthetic peptide HIV 1 O subgroup or a combination thereof; and recombinant antigen HIV 2 gp 36 antigen or synthetic peptide of HIV2 gp 36 or a combination thereof. The diagnostic kit includes microplate immobilized with HIV antigen , enzyme conjugate, conjugate diluent, luminol substrate, substrate diluent, positive control, negative control, calibrator, wash buffer and sample diluent. IN-KOL-2010-00719A