螺旋霉素高产菌株的选育

摘要

以螺旋霉素链霉菌(Streptomyces spiramyceticus)SPM-14078菌株作为出发菌株,利用常压室温等离子体(ARTP)诱变、紫外线(UV)诱变和亚硝基胍(NTG)诱变的方法对菌株进行诱变处理,比较3种方法的诱变效果,通过诱变筛选出1株遗传稳定性好且具有Co2+和豆油耐受性、噬菌体抗性的菌株SPMAR-1603,其发酵效价比出发菌株提高了294.9%,此结果对螺旋霉素高效生产、品质提升具有重要意义.%In order to screening of high yield strain for spiramycin,Streptomyces spiramyceticus SPM-14078 strain was selected as original strain. The original strain was mutation treated with atmospheric and room temperature plasmas(ARTP),Ultraviolet(UV)and nitrosoguanidine(NTG),respectively. The mutagenic effects of the three methods were compared. The SPMAR-1603 strain with high inheritance stability, Co2+tolerance and phage resistance,was screened. The potency of SPMAR-1603 strain had improved 294.9% than the original strain. The result had great significance for efficient production and quality improvement of spiramycin.