应用超声微泡对正常和不同程度缺血心肌微循环血流速度及血流状态的研究

摘要

Objective The study aims to investigate blood flow velocity and blood flow status of microcirculation in normal myocardium, ischemic myocardium by using ultrasound microbubbles. Methods Blood flow velocity of normal microcirculation in myocardium was determined, using DFY- Ⅱ Ultrasound Imaging Analysis Software among 15 experimental dogs. Dog models of myocardial ischemia with coronary stenoses of different degrees were established; blood flow status in ischemic myocardium was observed, and blood flow velocity was measured. Results The blood flow velocities in normal myocardium was 6.25±1.27 mm/s. In dogs with 50％, 90 ％, and 100 ％ myocardial ischemia, the myocardial blood flow velocities were 2. 3±0. 57 mm/s,0. l±0. 04 mm/s, and 0. 05±0. 01 mm/s, respectively. Blood flow gradually slowed down as the artificial stenosis aggravated (P＜0. 05). Conclusions A noninvasive, dynamic real time, simple, and direct method to determine microcirculation blood flow velocity in deep organs is successfully established.%目的 通过单个超声微泡研究正常及不同缺血程度下的心肌微循环血流速度及血流状态.方法 采用15只实验犬用"DFY型超声图像定量分析仪"观测心肌正常微循环的血流速度.建立不同程度冠脉狭窄的心肌缺血模型,并观测缺血心肌的血流状态及速度.结果 正常心肌微循环的血流速度为(6.25±1.27)mm/s,心肌50%、90%和100%缺血时心肌微循环的血流速度分别为(2.3±0.57)mm/s、(0.1±0.04)mm/s和(0.05±0.01)mm/s.随冠脉狭窄程度加大血流速度明显减慢(P＜0.05).结论 本实验所用方法是一种无创、动态实时、直接测量心肌微循环血流速度的新技术.