密叶铁线蕨的离体快繁研究

摘要

The sporophyllary leaves of Adiantum raddianum Fritz-Luethii were used as explants to study the process of rapid propagation in vitro. The results showed that the rate of the spore germination was 95.6% in 1/2 MS culture medium with 3 mg/L GA and the prothallus propagated at a speed of over 4 times in 1/2 MS culture medium without plant growth hormone. Sporophyte formation was almost impossible in tissue culture, but when prothallia were mixed with different solution and comminuted in a waring blender, and applied to soil, every group of prothallia produced over 1 000 sporophytes.%利用密叶铁线蕨(A diantum raddianum Fritz-Luethii)孢子叶为外植体进行离体快繁研究.结果表明,密叶铁线蕨的成熟孢子在附加GA 3 mg/L的1/2 MS培养基上培养,其萌发率可达95.6％,产生的原叶体在不含激素的1/2 MS培养基上继代,繁殖倍数达4倍以上,但孢子体难以在组培过程中直接获得;将组培过程产生的原叶体从培养瓶内取出后与1/2 MS改良盐溶液混合搅碎,播于基质上,在温室大棚中培养,每团原叶体可产生孢子体超过1 000个.