糯米香种质资源遗传多样性的RAPD分析

摘要

Genetic diversity of twelve accessions of Strobilanthes tonkinensis was evaluated by RAPD. The results showed that 305 bands were amplified by 41 random primers, among which 120 were polymorphic, accounting for 39.34% of the total bands, which revealed a low degree of genetic diversity among the twelve accessions. The genetic similarity analysis demonstrated that their genetic similarity coefficients were between 0.734 4 and 0.963 9. The phylogenetic dendrogram with UPGMA revealed that the twelve accessions could be divided into two clusters I and II (D=0.821). More over, the germplasm resources coming from the same geographic area presented closer relationship in phylogeny.%利用RAPD技术,对12份糯米香种质资源进行遗传多样性研究.从95条随机引物中筛选出41条条带清晰、重复性及多态性好的引物,对12份糯米香种质资源的基因组DNA进行PCR扩增,共扩增出305条带,其中扩增条带的多态位点有120个,在物种水平上,多态性位点百分率为39.34％,表明供试糯米香种质资源遗传多样性较低.遗传相似性在0.734 4～0.963 9之间,12份种质表现出较高的遗传相似性.通过UPGMA构建树状图,当相似系数为0.821时,12份种质资源可以聚为I和Ⅱ两个类群,在地理分布图上,来源地相同或较近的种质表现出较为亲密的亲缘关系.