福建东南部野生蕉7个自然居群289份样品的ISSR分析

摘要

The genomic DNAs of 289 samples of the wild bananas from 7 populations in southeastern Fujian were extracted from the leaves for ISSR analyses. The results showed that: ① 16 primers were screened from 40 arbitrary primers, and a total of 174 bands were amplified from the 289 samples, among which 121 (69.54%)were polymorphic. ②the PPB of 7 populations of the wild banana resources were betweenl7.24%~45.4%, which were all at the lower levels but remarkably different among them. ③The inter-populations variance was 58.24% and inside-population variance was 41.76%. ④The cluster analysis results of ISSR markers indicated that they had a close relationship between genetic relationship and geography.%对福建东南部7个野生蕉自然居群289份样品进行ISSR及聚类分析.结果表明:①16条ISSR引物共扩增出条带174条,其中多态性条带121条,多态性百分率(P)69.54％.②7个野生蕉自然居群的多态位点百分率为17.24％～45.4％,且差异显著,但遗传多样性水平均不高.③总的遗传变异中,58.24％基因变异来源于各野生蕉居群之间,41.76％基因变异来源于各个野生蕉居群内部交流.7个野生蕉居群间基因流较低,不能维持较高的基因变异多样性.④聚类分析结果表明,其亲缘关系与地理分布有密切关系.