脑宁康颗粒对脑缺血预适应和脑缺血再灌注大鼠血小板膜表面受体水平的影响

摘要

BACKGROUND: As molecular marker of platelet, CD62P not only reflects activated degree and functional status of platelet but also mediates adherent function of platelet with neutrophilic granulocyte and endothelial cell so as to deteriorate reperfusion injury after cerebral ishcemia.OBJECTIVE: To observe effect of naoningkang (NNK) granules on CD62P level of platelet in rats with cerebral ischemic preconditioning and cerebral ischemia-reperfusion and compare with aspirin at the same time.DESIGN: Randomized control study.SETTING: Encephalopathy Center of Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine.MATERIALS: The experiment was completed in Shandong University from July 1998 to February 1999. Totally 48 male Wistar males were selected from Experimental Animal Center of Shandong University. All rats were randomly divided into model group, sham operation group, aspirin group and NNK group with 12 in each group. Six rats were selected from each group for modeling with cerebral ischemic preconditioning method, and other 6 with ischemia-reperfusion method. NNK granules were composed of chuanxiong, flea and seaweed, and contained 5 g/g dried medicinal herbs.METHODS: Ischemic preconditioning (IPC) model and cerebral ischemiareperfusion model were made directed according to Pulsinelli four tubes method and Liu's method respectively. Blood of rats were obtained under anesthesia after 24-hour ischemia-reperfusion. CD62P level on the membrane surface of platelet in rats with cerebral ischemic preconditioning and cerebral ischemia-reperfusion was assayed and compared with fluorescence antibody labelling method on flow cytometer.MAIN OUTCOME MEASURES: CD62P level of platelet in rats with cerebral ischemic preconditioning and cerebral ischemia-reperfusion.chemia-reperfusion, CD62P level of rats in the two model groups was increased obviously (P ＜ 0.01), but the increase in NNK group and aspirin mong cerebral ischemic preconditioning models showed less changes in the two kinds of models, both NNK and aspirin showed positive effect on CD62P, and the effect of NNK group was a little superior to that of aspirin group, but the difference was not significant (P ＞ 0.05).CONCLUSION: NNK has positive effect on CD62P expression in both cerebral ischemic preconditioning and cerebral ischemia-reperfusion models, and the effect of NNK group is a little superior to that of aspirin group,this suggests that NNK can inhibit its aggregation, improve local microcirculation of brain through interfering in activation of platelet so as to protect cerebral neurons.%背景:CD62p作为血小板活化的分子标记物,不仅反应了血小板活化程度及功能状态,还介导血小板与中性粒细胞及内皮细胞的粘附功能,加重脑缺血后再灌注损伤.目的:观察中药脑宁康颗粒对经脑缺血预适应和脑缺血再灌注处理后的大鼠血小板膜表面受体水平的影响,并与西药阿司匹林进行比较.设计:随机对照实验.单位:上海中医药大学附属曙光医院脑病中心.材料:实验于1998-07/1999-02在山东医科大学完成.选择雄性Wistar大鼠48只,随机分为模型组,假手术组,阿司匹林组和脑宁康组,每组12只.每组取6只用脑缺血预适应方法造模.其余6只用脑缺血再灌注方法造模.脑宁康颗粒由川芎,蚤休,海藻等成分组成,生药含量为5 g/g.方法:根据Pulsinelli四管法及Liu法分别制作大鼠脑缺血预适应和脑缺血再灌注模型,分别于缺血再灌注24 h后在麻醉状态下断头取血,以荧光抗体标记法在流式细胞仪上测定并比较脑缺血预适应和脑缺血再灌注大鼠血小板膜表面受体水平.主要观察指标:脑缺血预适应和脑缺血再灌注大鼠血小板膜表面受体水平.结果:48只大鼠均进入结果分析.①缺血再灌注后24 h,两种方法模型组大鼠的CD 62P值均明显升高(P＜0.01),而脑宁康和阿司匹林治疗组CD 62P虽有增加,但不明显(P＞0.05).②脑缺血预适应模型中除假手术组外,各组CD 62P值均较脑缺血再灌注模型变化幅度小.③在两种模型中,脑宁康颗粒和阿司匹林均对CD 62P有良性效应,其中脑宁康组略优于阿司匹林组,但差异无显著性意义(P＞0.05).结论:脑宁康颗粒对脑缺血再灌注和脑缺血预适应状态下的CD 62P表达均有良性效应,其强度略高于阿司匹林,说明脑宁康颗粒通过干扰血小板活化,抑制其聚集、改善脑局部微循环,从而保护脑神经元.