中国汉族、维吾尔族、哈萨克族、蒙古族健康绝经后妇女维生素D受体基因多态性的分布

摘要

背景:维生素D受体基因在内切酶Bsm Ⅰ,ApaⅠ,TaqⅠ作用下,呈限制性内切酶片段长度多态性,并且与骨密度密切相关,而骨密度的变化对骨质疏松起着重要作用,但维生素D受体基因多态性与骨密度、骨质疏松相关性尚无定论.目的:分析在中国汉族、维吾尔族、哈萨克族、蒙古族绝经后妇女中与骨密度密切相关的维生素D受体基因多态性分布规律.设计:对比观察.单位:解放军总医院老年医学研究所.对象:选择2002-01/2003-12在解放军总医院进行健康体检的汉族绝经后妇女179名,平均年龄(59±3)岁.选择2001-01/2003-12于解放军兰州军区乌鲁木齐总医院老年科进行健康体检的维吾尔族、哈萨克族、蒙古族绝经后妇女者122,63,112名,平均年龄分别为(56±4),(55±3),(57±3)岁.均对检测项目知情同意.方法:应用聚合酶链反应限制性片段长度多态性技术确定维生素D受体基因BsmⅠ基因型,分析汉族、维吾尔族、哈萨克族和蒙古族绝经后妇女维生素D受体基因BsmⅠ多态性分布频率,并与已知的美国、澳大利亚、法国和日本相应数据进行比较.计数资料差异比较采用χ2检验.主要观察指标:汉族、维吾尔族、哈萨克族和蒙古族绝经后妇女维生素D受体基因BsmⅠ多态性分布频率,以及该分布特点与美国、澳大利亚、法国和日本相应数据比较结果.结果:汉族、维吾尔族、哈萨克族和蒙古族绝经后妇女维生素D受体基因bb基因型频率分别为90.5%,69.67%,38.1%和50%,BB基因型频率分别为0,4.1%,6.35%和4.46%,汉族与维吾尔族、哈萨克族和蒙古族维生素D受体基因型频率分布比较,差异明显(P＜0.01).哈萨克族与欧美人种比较,维生素D受体基因型差异不明显,与日本、韩国人种差异明显(P＜0.01).结论:中国汉族与维吾尔族、哈萨克族和蒙古族绝经后妇女维生素D受体基因型多态性具明显种族差异性;哈萨克族维生素D受体基因型频率分布接近欧美人种,与日本、韩国人种差异明显.%BACKGROUND: Vitamin D receptor (VDR) gene shows restriction fragment length polymorphism with incision enzyme Bsm Ⅰ ,Apa Ⅰ ,Taq Ⅰ ,which is related to bone mineral density (BMD).However, it is unclear that the relationship between VDR gene (Bsm Ⅰ ) polymorphisms and BMD,osteoporosis.OBJECTIVE: To analyze the distribution regularity of vitamin D receptor gene polymorphism related to BMD in postmenopausal women of Han,Uygur, Kazak and Mongoloid nationality in China. DESIGN: controlled observation.SETTING: Institute of Gerontology,General Hospital of Chinese PLA.PARTICIPANTS: Totally 179 women of Han,who were taking physical examination in General Hospital of Chinese PLA from January 2002 to December 2003, at the average age of (59±3) years,were selected. A total number of 122 women of Uygur with average age of 56.49 years; 63 women of Kazak with average age of (55±3) years; and 112 women of Mongoloid with average age of (57±3) years,who were all taking physical examination in department of geriatrics, Urumqi General Hospital of Lanzhou Military Area Command of Chinese PLA from January 2001 to December 2003.All of them were informed consent.METHODS: VDR genotypes(Bsm Ⅰ ) were defined with polymerase chain reaction-restriction fragment length polymorphism,so as to analyze distribution of Vitamin-D receptor gene (Bsm Ⅰ )polymorphisms of postmenopausal women in Han,Uygur, Kazak,Mongoloid nationality,and compared with the data of USA,Australia,France,Japan,Korea. Enumeration data were compared with Chi-square test.MAIN OUTCOME MEASURES: VDR (Bsm Ⅰ ) polymorphisms in healthy postmenopausal women from Han, Uygur, Kazak, Mongoloid populations in China, which were compared with the data of USA, Australia,France, Japan, Korea.RESULTS: For women of Han, Uygur, Kazak and Mongoloid nationality,the BB genotypes accounted for 0, 4.1%, 6.35% and 4.46%, the bb genotypes accounted for 90.5%, 69.67%, 38.1% and 50% respectively. There was a significant difference between women of Han, Uygur, Kazak, and Mongoloid nationality(P ＜ 0.01). There was insignificant difference in comparation of distribtuion of VDR genotype between Kazak nationality and the west races, but it was significantly different to that in Japan,Korea races.CONCLUSION:VDR genotype polymorphisms is characterized by obvious racial diversify in postmenopausal women of Han,Uygur, Kazak,Mongoloid populations in China;Distribution of VDR gene frequency of Kazak population is similar with the west race ,but is different to Japanese and Korea's race.