当归补血汤载药血清干预后骨髓基质细胞中粒细胞集落刺激因子和白细胞介素3的分泌

摘要

背景:实验表明,当归补血汤的有效成分多糖对造血干细胞和造血祖细胞的增殖分化存在显著的促进作用,对造血微环境具有重要的调控作用.目的:观察当归补血汤载药血清干预后小鼠骨髓基质细胞中粒细胞集落刺激因子和白细胞介素3的分泌状况.方法:分离骨髓基质细胞,于96孔板培养,在相差显微镜下观察细胞形态和生长状况;将细胞分4组,加含有不同剂量的当归补血汤载药血清进行干预,采用MTT法检测各组细胞的增殖活性,ELISA法检测粒细胞集落刺激因子和白细胞介素3的表达情况.结果与结论:当归补血汤载药血清可明显促进骨髓基质细胞的增殖,促进骨髓基质细胞分泌粒细胞集落刺激因子和白细胞介素3,呈剂量依赖性,含等效剂量载药血清组促增殖作用和促分泌作用优于其他各组,浓度过高可减弱细胞的增殖分泌效应.%BACKGROUND: Experiment suggests that the active component of Danggui Buxue decoction, i.e. polysaccharides, can significantly promote proliferation and differentiation of hematopoietic stem cells and hematopoietic progenitor cells, and regulate hematopoietic microenvironment.OBJECTIVE: To investigate the effect of serum containing Danggui Buxue decoction on granulocyte colony-stimulating factor (G-CSF) and interleukin-3 (IL-3) secretion in mice bone marrow stromal cells (BMSCs). METHODS: BMSCs were isolated and cultured in 96-well culture plate. BMSCs morphology and growth were observed by phase contrast microscopy. The cells were divided into four groups and cultured with different concentrations of the serum containing Danggui Buxue decoction. MTT method was used to observe the proliferation of BMSCs, and ELISA method was used to observe the expression level of G-CSF and IL-3. RESULTS AND CONCLUSION: Serum containing Danggui Buxue decoction significantly promoted BMSCs proliferation, and increased the expression level of G-CSF and IL-3 secreted from BMSCs in a dose-dependent manner. Notably, serum containing equivalent dose drugs significantly promoted BMSCs proliferation, and G-CSF and IL-3 expression compared with other groups. Higher concentration weakens cell proliferation and secretion effects.