基质细胞衍生因子1及其受体CXCR4在内源性神经干细胞迁移中的作用

摘要

BACKGROUND: Neural stem cells own the characteristics of migration in vitro. However, the mechanisms underlying the migration of these cells remain unclear.OBJECTIVE: To investigate the effects of stromal cell-derived factor-1 and its receptor CXCR4 on the migration of neuralstem cells in vivo.METHODS: The brain injured rat model was established with cortical micro-injection of lipopolysaccharide. At 3, 5, and 7 daysafter modeling, the brain tissue was harvested. Immunohistochemical staining of frozen section was used to detect theexpression of stromal cell-derived factor-1 and nestin-positive cells in different times and phages.RESULTS AND CONCLUSION: Immunohistochemical staining showed that the expression of stromal cell-derivedfactor-1 was in a time-dependent manner. Nestin-positive cells extended and migrated outward significantly and pointedto the lipopolysaccharide injection area. Stromal cell-derived factor-1 can induce the endogenous neural stem cells tomove towards lipopolysaccharide injection area in vivo.%背景:神经干细胞具有体外、体外迁移的特性,但关于其迁移的具体机制还不十分清楚.目的:探讨基质细胞衍生因子1及其受体CXCR4在神经干细胞体内迁移中的作用.方法:制备脑皮质微量注射脂多糖脑损伤大鼠模型,于建模后3,5,7 d灌注取脑,采用冰冻切片免疫组织化学方法,动态监测基质细胞衍生因子1和nestin表达的时相变化.结果与结论:免疫组织化学染色观察显示:基质细胞衍生因子1随时间推移表达的量逐渐增多.Nestin阳性细胞有明显向外延伸和迁移的迹象,尖端指向脂多糖注射区.结果可见基质细胞衍生因子1趋化体内表达其特异性受体的内源性神经干细胞向脂多糖注射区迁移.